Perifollicular blood flow Doppler indices, but not follicular pO2, pCO2, or pH, predict oocyte developmental competence in in vitro fertilization

Suzanne Huey, Alfred Abuhamad, Gerardo Barroso, Ming I. Hsu, Paul Kolm, Jacob Mayer, Sergio Oehninger

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

Objective: To assess the relationships among perifollicular blood flow; follicular fluid pO2, pCO2, and pH; oocyte developmental capacity; preimplantation embryo quality. Design: Prospective study. Setting: Academic, tertiary care institution. Patient(s): Unselected, gonadotropin-stimulated IVF cycles. Intervention(s): Color, pulsed Doppler analysis of perifollicular blood flow, and follicular pO2, pCO2, and pH determinations of randomly designated, mapped ovarian follicles. Main Outcome Measure(s): Fertilization and day 3 embryo cleavage and morphology. Result(s): Perifollicular vascularity indices were significantly and negatively correlated with day 3 embryo cleavage. Pulsatility index and S-D ratio also were significantly and negatively correlated with follicular pO2. The same correlation was found between resistance index and the fertilization rate of preovulatory oocytes. No relationship existed between follicular metabolic analysis and fertilization or embryo quality. The resistance index had a sensitivity of 0.57 and a specificity of 0.71 for the prediction of advanced embryo cleavage status. Conclusion(s): Results confirm and extend previous reports demonstrating that color, pulsed Doppler ultrasound analysis of individual preovulatory follicles during IVF therapy may provide an indirect index of the developmental competence of the corresponding oocyte. Although these methods may provide means to select embryos for transfer with the highest implantation potential, the moderate predictive power showed so far may limit their clinical applicability.

Original languageEnglish
Pages (from-to)707-712
Number of pages6
JournalFertility and Sterility
Volume72
Issue number4
DOIs
Publication statusPublished - Oct 1999
Externally publishedYes

Fingerprint

Fertilization in Vitro
Mental Competency
Oocytes
Embryonic Structures
Fertilization
Color
Doppler Ultrasonography
Follicular Fluid
Ovarian Follicle
Embryo Transfer
Blastocyst
Tertiary Healthcare
Gonadotropins
Outcome Assessment (Health Care)
Prospective Studies
Therapeutics

Keywords

  • Blood flow
  • Doppler analysis
  • Embryo cleavage
  • Follicular fluid
  • IVF
  • Oocyte development
  • PH
  • PO
  • Predictive value

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

Cite this

Perifollicular blood flow Doppler indices, but not follicular pO2, pCO2, or pH, predict oocyte developmental competence in in vitro fertilization. / Huey, Suzanne; Abuhamad, Alfred; Barroso, Gerardo; Hsu, Ming I.; Kolm, Paul; Mayer, Jacob; Oehninger, Sergio.

In: Fertility and Sterility, Vol. 72, No. 4, 10.1999, p. 707-712.

Research output: Contribution to journalArticle

Huey, Suzanne ; Abuhamad, Alfred ; Barroso, Gerardo ; Hsu, Ming I. ; Kolm, Paul ; Mayer, Jacob ; Oehninger, Sergio. / Perifollicular blood flow Doppler indices, but not follicular pO2, pCO2, or pH, predict oocyte developmental competence in in vitro fertilization. In: Fertility and Sterility. 1999 ; Vol. 72, No. 4. pp. 707-712.
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AU - Huey, Suzanne

AU - Abuhamad, Alfred

AU - Barroso, Gerardo

AU - Hsu, Ming I.

AU - Kolm, Paul

AU - Mayer, Jacob

AU - Oehninger, Sergio

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AB - Objective: To assess the relationships among perifollicular blood flow; follicular fluid pO2, pCO2, and pH; oocyte developmental capacity; preimplantation embryo quality. Design: Prospective study. Setting: Academic, tertiary care institution. Patient(s): Unselected, gonadotropin-stimulated IVF cycles. Intervention(s): Color, pulsed Doppler analysis of perifollicular blood flow, and follicular pO2, pCO2, and pH determinations of randomly designated, mapped ovarian follicles. Main Outcome Measure(s): Fertilization and day 3 embryo cleavage and morphology. Result(s): Perifollicular vascularity indices were significantly and negatively correlated with day 3 embryo cleavage. Pulsatility index and S-D ratio also were significantly and negatively correlated with follicular pO2. The same correlation was found between resistance index and the fertilization rate of preovulatory oocytes. No relationship existed between follicular metabolic analysis and fertilization or embryo quality. The resistance index had a sensitivity of 0.57 and a specificity of 0.71 for the prediction of advanced embryo cleavage status. Conclusion(s): Results confirm and extend previous reports demonstrating that color, pulsed Doppler ultrasound analysis of individual preovulatory follicles during IVF therapy may provide an indirect index of the developmental competence of the corresponding oocyte. Although these methods may provide means to select embryos for transfer with the highest implantation potential, the moderate predictive power showed so far may limit their clinical applicability.

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