Periaqueductal gray glutamatergic transmission governs chronic stress-induced depression

Yu Cheng Ho, Tzer Bin Lin, Ming Chun Hsieh, Cheng Yuan Lai, Dylan Chou, Yat Pang Chau, Gin Den Chen, Hsien Yu Peng

Research output: Contribution to journalArticle

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Abstract

The mechanisms underlying chronic stress-induced dysfunction of glutamatergic transmission that contribute to helplessness-associated depressive disorder are unknown. We investigated the relationship of á-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors and stress, and the neuroplastic changes of stress-induced depression-like behavior in the ventrolateral periaqueductal gray (vlPAG). We conducted whole-cell patch-clamp electrophysiological recordings in the vlPAG neurons. Depression-like behavior was assayed using tail suspension test and sucrose preference test. Surface and cytosolic glutamate receptor 1 (GluR1) AMPA receptor expression was analyzed using western blotting. Phosphorylated GluR1 expression was quantified using western blotting and immunohistochemical analysis. Unpredictable inescapable foot shock stress caused reduction in glutamatergic transmission originating from both presynaptic and postsynaptic loci in the vlPAG that was associated with behavioral despair and anhedonia in chronic stress-induced depression. Pharmacological inhibition of GluR1 function in the vlPAG caused depression-like behavior. Diminished glutamatergic transmission was due to reduced glutamate release presynaptically and enhanced GluR1-endocytosis from the cell surface postsynaptically. Chronic stress-induced neuroplastic changes and maladaptive behavior were reversed and mimicked by administration of glucocorticoid receptor (GR) antagonist and agonist, respectively. However, chronic stress did not affect γ-aminobutyric acid (GABA)-mediated inhibitory synaptic transmission in the vlPAG. These results demonstrate that depression-like behavior is associated with remarkable reduction in glutamatergic, but not GABAergic, transmission in the vlPAG. These neuroplastic changes and maladaptive behavior are attributed to GRdependent mechanisms. As reduced GluR1-associated responses in the vlPAG contribute to chronic stress-induced neuroplastic changes, this cellular mechanism may be a critical component in the pathogenesis of stress-associated neuropsychiatric disorders.

Original languageEnglish
Pages (from-to)302-312
Number of pages11
JournalNeuropsychopharmacology
Volume43
Issue number2
DOIs
Publication statusPublished - Jan 1 2018

Fingerprint

Periaqueductal Gray
Glutamate Receptors
Depression
AMPA Receptors
Western Blotting
Anhedonia
Hindlimb Suspension
Aminobutyrates
Glucocorticoid Receptors
Depressive Disorder
Endocytosis
Synaptic Transmission
gamma-Aminobutyric Acid
Sucrose
Foot
Glutamic Acid
Shock
Pharmacology
Neurons
Acids

ASJC Scopus subject areas

  • Pharmacology
  • Psychiatry and Mental health

Cite this

Periaqueductal gray glutamatergic transmission governs chronic stress-induced depression. / Ho, Yu Cheng; Lin, Tzer Bin; Hsieh, Ming Chun; Lai, Cheng Yuan; Chou, Dylan; Chau, Yat Pang; Chen, Gin Den; Peng, Hsien Yu.

In: Neuropsychopharmacology, Vol. 43, No. 2, 01.01.2018, p. 302-312.

Research output: Contribution to journalArticle

Ho, YC, Lin, TB, Hsieh, MC, Lai, CY, Chou, D, Chau, YP, Chen, GD & Peng, HY 2018, 'Periaqueductal gray glutamatergic transmission governs chronic stress-induced depression', Neuropsychopharmacology, vol. 43, no. 2, pp. 302-312. https://doi.org/10.1038/npp.2017.199
Ho, Yu Cheng ; Lin, Tzer Bin ; Hsieh, Ming Chun ; Lai, Cheng Yuan ; Chou, Dylan ; Chau, Yat Pang ; Chen, Gin Den ; Peng, Hsien Yu. / Periaqueductal gray glutamatergic transmission governs chronic stress-induced depression. In: Neuropsychopharmacology. 2018 ; Vol. 43, No. 2. pp. 302-312.
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