Performance of the BDProbeTec ET Assay for Identification Of Mycobacterium Tuberculosis from Clinical Isolates

許 至仁(Chih-Jen Hsu), 白 冠壬(Kuan-Jen Bai), 周 梓光(Tzu-Kuang Chou), 江 振源(Chen-Yuan Chiang), 索 任(Suo Jen)

Research output: Contribution to journalArticle

Abstract

To assess the performance of the BDProbeTec assay in the identification of Mycobacterium tuberculosis, 60 cultures of Mycobacterium from respiratory specimens were tested with M. tuberculosis complex probes. Using conventional biochemical tests, 30 of them were determined to be M. tuberculosis and 30 nontuberculous mycobacteria (NTM) (13 M. avium complex, 7 M. abscessus, 2 M. fortuitum, 2 M. kansasii, 2 M. phlei, 2 M. terrae, 1 M. simiae, and 1 M. vaccae). The BDProbeTec detected 100% (30) of the M. tuberculosis isolates, while 96.7% (29) of the NTM isolates tested negative. Only 1 NTM isolate, which was M. phlei, showed a positive result. The sensitivity, specificity, positive predictive value, and negative predictive value were 100%, 96.7%, 96.7%, and 100%, respectively. We conclude that the BDProbeTec ET system is a robust assay for the identification of M. tuberculosis from mycobacterium cultures. Determining the reliability of the BDProbeTec system for the direct detection of M. tuberculosis in respiratory specimens requires further study.
Original languageEnglish
Pages (from-to)232-237
Number of pages6
Journal胸腔醫學
Volume17
Issue number3
Publication statusPublished - 2002

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Mycobacterium
Mycobacterium tuberculosis
assays
Mycobacterium tuberculosis complex
testing

Keywords

  • 結核分枝桿菌
  • 非結核分枝桿菌
  • BDProbeTec ET
  • M. tuberculosis
  • Nontuberculous Mycobacteria

Cite this

Performance of the BDProbeTec ET Assay for Identification Of Mycobacterium Tuberculosis from Clinical Isolates. / 許至仁(Chih-Jen Hsu); 白冠壬(Kuan-Jen Bai); 周梓光(Tzu-Kuang Chou); 江振源(Chen-Yuan Chiang); 索任(Suo Jen).

In: 胸腔醫學, Vol. 17, No. 3, 2002, p. 232-237.

Research output: Contribution to journalArticle

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AU - 周, 梓光(Tzu-Kuang Chou)

AU - 江, 振源(Chen-Yuan Chiang)

AU - 索, 任(Suo Jen)

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