To assess the performance of the BDProbeTec assay in the identification of Mycobacterium tuberculosis, 60 cultures of Mycobacterium from respiratory specimens were tested with M. tuberculosis complex probes. Using conventional biochemical tests, 30 of them were determined to be M. tuberculosis and 30 nontuberculous mycobacteria (NTM) (13 M. avium complex, 7 M. abscessus, 2 M. fortuitum, 2 M. kansasii, 2 M. phlei, 2 M. terrae, 1 M. simiae, and 1 M. vaccae). The BDProbeTec detected 100% (30) of the M. tuberculosis isolates, while 96.7% (29) of the NTM isolates tested negative. Only 1 NTM isolate, which was M. phlei, showed a positive result. The sensitivity, specificity, positive predictive value, and negative predictive value were 100%, 96.7%, 96.7%, and 100%, respectively. We conclude that the BDProbeTec ET system is a robust assay for the identification of M. tuberculosis from mycobacterium cultures. Determining the reliability of the BDProbeTec system for the direct detection of M. tuberculosis in respiratory specimens requires further study.
|Number of pages||6|
|Publication status||Published - 2002|
- BDProbeTec ET
- M. tuberculosis
- Nontuberculous Mycobacteria
許至仁(Chih-Jen H, 白冠壬(Kuan-Jen B, 周梓光(Tzu-Kuang C, 江振源(Chen-Yuan C, & 索任(Suo J (2002). Performance of the BDProbeTec ET Assay for Identification Of Mycobacterium Tuberculosis from Clinical Isolates. 胸腔醫學, 17(3), 232-237. http://www.AiritiLibrary.com/Publication/Index/10239855-200209-17-3-232-237-a