大豆煮汁的胃蛋白酶水解物中具有抑制血管收縮素轉換酵素之勝肽

Translated title of the contribution: Peptides with angiotensin I converting enzyme inhibitory activity in pepsin-digests of soybean broth

J. R. Chen, S. C. Yang, Y. L. Liu, K. Suetsuna, M. J. Shieh

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Eight inhibitory peptides of angiotensin I converting enzyme (ACE) were isolated from the hydrolysate of soybean broth. The amino acid sequences of these peptides identified by Edman procedure were: Val-Thr-Val-Pro-Gln, Val- Val-Phe-Asp, Gly-Glu-Leu-Phe-Glu, Ile-Thr-Pro-Leu, Gln-Val-Val-Phe, Val-Gln- Val-Val-Phe, Ile-Val-Phe-Asp-Ala and Gly-Asp-Ala-Pro-Asn. The IC50 value of these peptides for ACE from bovine lung were: 6.3, 3.9, 2.3, 5.2, 4.6, 3.4, 4.1 and 2.8 x 10-5 M, respectively. These peptides contained hydrophobic and aromatic amino acids. This characteristic, was considered to be highly related to their inhibitory effect on ACE activity.

Original languageTraditional Chinese
Pages (from-to)435-444
Number of pages10
JournalNutritional Sciences Journal
Volume22
Issue number4
Publication statusPublished - 1997

Fingerprint

Pepsin A
peptidyl-dipeptidase A
Peptidyl-Dipeptidase A
pepsin
Soybeans
peptides
soybeans
Peptides
Aromatic Amino Acids
hydrolysates
Inhibitory Concentration 50
aromatic compounds
inhibitory concentration 50
Amino Acid Sequence
amino acid sequences
lungs
enzyme activity
Lung
amino acids
cattle

Keywords

  • Angiotensin I converting enzyme
  • Peptide
  • Soybean
  • Soybean protein

ASJC Scopus subject areas

  • Food Science
  • Medicine (miscellaneous)

Cite this

大豆煮汁的胃蛋白酶水解物中具有抑制血管收縮素轉換酵素之勝肽. / Chen, J. R.; Yang, S. C.; Liu, Y. L.; Suetsuna, K.; Shieh, M. J.

In: Nutritional Sciences Journal, Vol. 22, No. 4, 1997, p. 435-444.

Research output: Contribution to journalArticle

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title = "大豆煮汁的胃蛋白酶水解物中具有抑制血管收縮素轉換酵素之勝肽",
abstract = "本研究從大豆煮汁的酵素分解物中分離出8種具有抑制血管收縮素轉換酵素活性的勝肽。經一次構造解析結果,這些勝肽分別為:Val-Thr-Val-Pro-Gln, Val-Val-Phe-Asp, Gly-Glu-Leu-Phe-Glu, Ile-Thr-Pro-Leu, Gin-Val-Val-Phe, Val-Gln-Val-Val-Phe, Ile-Val-Phe-Asp-Ala和Gly-Asp-Ala-Pro-Asn,半數有效濃度值(IC50)分別為6.3, 3.9, 2.3, 5.2, 4.6, 3.4, 4.1及2.8×10^(-5)M,其中以Gly-Glu-Leu-Phe-Glu的2.3×10^(-5)M為最低。具有抑制血管收縮素轉換酵素活性的勝肽中,多含有疏水性及芳香族胺基酸,該特性與活性的表現有密切的關連性。",
keywords = "Angiotensin I converting enzyme, Peptide, Soybean, Soybean protein, 大豆, 大豆蛋白質, 勝肽, 血管收縮素轉換酵素",
author = "Chen, {J. R.} and Yang, {S. C.} and Liu, {Y. L.} and K. Suetsuna and Shieh, {M. J.}",
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T1 - 大豆煮汁的胃蛋白酶水解物中具有抑制血管收縮素轉換酵素之勝肽

AU - Chen, J. R.

AU - Yang, S. C.

AU - Liu, Y. L.

AU - Suetsuna, K.

AU - Shieh, M. J.

PY - 1997

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N2 - 本研究從大豆煮汁的酵素分解物中分離出8種具有抑制血管收縮素轉換酵素活性的勝肽。經一次構造解析結果,這些勝肽分別為:Val-Thr-Val-Pro-Gln, Val-Val-Phe-Asp, Gly-Glu-Leu-Phe-Glu, Ile-Thr-Pro-Leu, Gin-Val-Val-Phe, Val-Gln-Val-Val-Phe, Ile-Val-Phe-Asp-Ala和Gly-Asp-Ala-Pro-Asn,半數有效濃度值(IC50)分別為6.3, 3.9, 2.3, 5.2, 4.6, 3.4, 4.1及2.8×10^(-5)M,其中以Gly-Glu-Leu-Phe-Glu的2.3×10^(-5)M為最低。具有抑制血管收縮素轉換酵素活性的勝肽中,多含有疏水性及芳香族胺基酸,該特性與活性的表現有密切的關連性。

AB - 本研究從大豆煮汁的酵素分解物中分離出8種具有抑制血管收縮素轉換酵素活性的勝肽。經一次構造解析結果,這些勝肽分別為:Val-Thr-Val-Pro-Gln, Val-Val-Phe-Asp, Gly-Glu-Leu-Phe-Glu, Ile-Thr-Pro-Leu, Gin-Val-Val-Phe, Val-Gln-Val-Val-Phe, Ile-Val-Phe-Asp-Ala和Gly-Asp-Ala-Pro-Asn,半數有效濃度值(IC50)分別為6.3, 3.9, 2.3, 5.2, 4.6, 3.4, 4.1及2.8×10^(-5)M,其中以Gly-Glu-Leu-Phe-Glu的2.3×10^(-5)M為最低。具有抑制血管收縮素轉換酵素活性的勝肽中,多含有疏水性及芳香族胺基酸,該特性與活性的表現有密切的關連性。

KW - Angiotensin I converting enzyme

KW - Peptide

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KW - Soybean protein

KW - 大豆

KW - 大豆蛋白質

KW - 勝肽

KW - 血管收縮素轉換酵素

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