Penaeus monodon caspase is targeted by a white spot syndrome virus anti-apoptosis protein

Jiann Horng Leu, Hao Ching Wang, Guang Hsiung Kou, Chu Fang Lo

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Caspases play a central and evolutionarily conserved role in mediating and executing apoptosis. Here, we report the cloning and characterization of a caspase from Penaeus monodon, Pm caspase. The full-length Pm caspase cDNA is 1386 bp, encoding a polypeptide of 304 amino acids with a calculated molecular mass of 34.3 kDa. BLASTP analysis against the NCBI nr database showed that Pm caspase is similar to insect effector caspases. RT-PCR analysis showed that Pm caspase mRNA is expressed in all examined tissues. When Pm caspase was overexpressed in SF-9 cells, the cells showed apoptotic morphological features, including the formation of apoptotic bodies and DNA ladders. The caspase-3 activity of Pm caspase was determined using the recombinant protein purified from Escherichia coli. Both RT-PCR and qRT-PCR analyses showed that the RNA levels of Pm caspase and P. monodon inhibitor of apoptosis protein (PmIAP) remained unchanged after white spot syndrome virus (WSSV) infection. We also used Pm caspase to show that WSSV449, an anti-apoptosis protein encoded by WSSV, is a direct caspase inhibitor.

Original languageEnglish
Pages (from-to)476-486
Number of pages11
JournalDevelopmental and Comparative Immunology
Volume32
Issue number5
DOIs
Publication statusPublished - 2008
Externally publishedYes

Fingerprint

White spot syndrome virus 1
Penaeidae
Caspases
Apoptosis
Proteins
Polymerase Chain Reaction
Effector Caspases
Inhibitor of Apoptosis Proteins
Caspase Inhibitors
Virus Diseases
Recombinant Proteins
Caspase 3
Insects
Organism Cloning

Keywords

  • Apoptosis
  • Caspase
  • IAP
  • Inhibitor of apoptosis protein
  • Penaeus monodon
  • White spot syndrome virus
  • WSSV
  • WSSV449

ASJC Scopus subject areas

  • Developmental Biology
  • Immunology

Cite this

Penaeus monodon caspase is targeted by a white spot syndrome virus anti-apoptosis protein. / Leu, Jiann Horng; Wang, Hao Ching; Kou, Guang Hsiung; Lo, Chu Fang.

In: Developmental and Comparative Immunology, Vol. 32, No. 5, 2008, p. 476-486.

Research output: Contribution to journalArticle

Leu, Jiann Horng ; Wang, Hao Ching ; Kou, Guang Hsiung ; Lo, Chu Fang. / Penaeus monodon caspase is targeted by a white spot syndrome virus anti-apoptosis protein. In: Developmental and Comparative Immunology. 2008 ; Vol. 32, No. 5. pp. 476-486.
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