PD-L1 Expression of Tumor Cells, Macrophages, and Immune Cells in Non–Small Cell Lung Cancer Patients with Malignant Pleural Effusion

Yen Han Tseng, Hsiang Ling Ho, Chiung Ru Lai, Yung Hung Luo, Yen Chiang Tseng, Jacqueline Whang-Peng, Yi hsuan Lin, Teh Ying Chou, Yuh Min Chen

Research output: Contribution to journalArticle

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Abstract

Introduction: Whether immunohistochemical staining of programmed death ligand 1 (PD-L1) on cells of pleural effusion could be used to predict response to immunotherapy treatment has not been reported. Methods: We retrospectively enrolled patients who had undergone malignant pleural effusion drainage and had effusion cell block specimens from 2014 to 2016. Immunohistochemical staining for PD-L1 was performed with tumor cells, immune cells, and macrophages of all cell block specimens. Immunoactivity was scored as 0 for absence of staining and 1+ for faint, 2+ for moderate, and 3+ for intense membranous staining. Patients’ clinicopathological characteristics were also collected. Results: PD-L1 expression of pleural effusion tumor cells was associated with the PD-L1 expression of macrophages (p = 0.003) and immune cells (p < 0.001). However, the PD-L1 expression of immune cells was not associated with that of macrophages. The PD-L1 expression of tumor cells was correlated with sex (p = 0.012), smoking status (p = 0.032), and Eastern Cooperative Oncology Group performance status (p = 0.017). The PD-L1 expression of immune cells was associated with the overall survival of patients (p = 0.004). Conclusions: These results suggest that there might be an immune interaction between pleural effusion tumor cells and macrophages. The low intensity of PD-L1 expression in immune cells is associated with the poor survival of patients with lung cancer with malignant pleural effusion.

Original languageEnglish
Pages (from-to)447-453
Number of pages7
JournalJournal of Thoracic Oncology
Volume13
Issue number3
DOIs
Publication statusPublished - Mar 1 2018

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Malignant Pleural Effusion
Non-Small Cell Lung Carcinoma
Macrophages
Ligands
Neoplasms
Pleural Effusion
Staining and Labeling
Survival
Immunotherapy
Drainage
Lung Neoplasms

Keywords

  • Immune cell
  • Lung cancer
  • Malignant pleural effusion
  • PD-L1
  • Tumor cell

ASJC Scopus subject areas

  • Oncology
  • Pulmonary and Respiratory Medicine

Cite this

PD-L1 Expression of Tumor Cells, Macrophages, and Immune Cells in Non–Small Cell Lung Cancer Patients with Malignant Pleural Effusion. / Tseng, Yen Han; Ho, Hsiang Ling; Lai, Chiung Ru; Luo, Yung Hung; Tseng, Yen Chiang; Whang-Peng, Jacqueline; Lin, Yi hsuan; Chou, Teh Ying; Chen, Yuh Min.

In: Journal of Thoracic Oncology, Vol. 13, No. 3, 01.03.2018, p. 447-453.

Research output: Contribution to journalArticle

Tseng, Yen Han ; Ho, Hsiang Ling ; Lai, Chiung Ru ; Luo, Yung Hung ; Tseng, Yen Chiang ; Whang-Peng, Jacqueline ; Lin, Yi hsuan ; Chou, Teh Ying ; Chen, Yuh Min. / PD-L1 Expression of Tumor Cells, Macrophages, and Immune Cells in Non–Small Cell Lung Cancer Patients with Malignant Pleural Effusion. In: Journal of Thoracic Oncology. 2018 ; Vol. 13, No. 3. pp. 447-453.
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AU - Ho, Hsiang Ling

AU - Lai, Chiung Ru

AU - Luo, Yung Hung

AU - Tseng, Yen Chiang

AU - Whang-Peng, Jacqueline

AU - Lin, Yi hsuan

AU - Chou, Teh Ying

AU - Chen, Yuh Min

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N2 - Introduction: Whether immunohistochemical staining of programmed death ligand 1 (PD-L1) on cells of pleural effusion could be used to predict response to immunotherapy treatment has not been reported. Methods: We retrospectively enrolled patients who had undergone malignant pleural effusion drainage and had effusion cell block specimens from 2014 to 2016. Immunohistochemical staining for PD-L1 was performed with tumor cells, immune cells, and macrophages of all cell block specimens. Immunoactivity was scored as 0 for absence of staining and 1+ for faint, 2+ for moderate, and 3+ for intense membranous staining. Patients’ clinicopathological characteristics were also collected. Results: PD-L1 expression of pleural effusion tumor cells was associated with the PD-L1 expression of macrophages (p = 0.003) and immune cells (p < 0.001). However, the PD-L1 expression of immune cells was not associated with that of macrophages. The PD-L1 expression of tumor cells was correlated with sex (p = 0.012), smoking status (p = 0.032), and Eastern Cooperative Oncology Group performance status (p = 0.017). The PD-L1 expression of immune cells was associated with the overall survival of patients (p = 0.004). Conclusions: These results suggest that there might be an immune interaction between pleural effusion tumor cells and macrophages. The low intensity of PD-L1 expression in immune cells is associated with the poor survival of patients with lung cancer with malignant pleural effusion.

AB - Introduction: Whether immunohistochemical staining of programmed death ligand 1 (PD-L1) on cells of pleural effusion could be used to predict response to immunotherapy treatment has not been reported. Methods: We retrospectively enrolled patients who had undergone malignant pleural effusion drainage and had effusion cell block specimens from 2014 to 2016. Immunohistochemical staining for PD-L1 was performed with tumor cells, immune cells, and macrophages of all cell block specimens. Immunoactivity was scored as 0 for absence of staining and 1+ for faint, 2+ for moderate, and 3+ for intense membranous staining. Patients’ clinicopathological characteristics were also collected. Results: PD-L1 expression of pleural effusion tumor cells was associated with the PD-L1 expression of macrophages (p = 0.003) and immune cells (p < 0.001). However, the PD-L1 expression of immune cells was not associated with that of macrophages. The PD-L1 expression of tumor cells was correlated with sex (p = 0.012), smoking status (p = 0.032), and Eastern Cooperative Oncology Group performance status (p = 0.017). The PD-L1 expression of immune cells was associated with the overall survival of patients (p = 0.004). Conclusions: These results suggest that there might be an immune interaction between pleural effusion tumor cells and macrophages. The low intensity of PD-L1 expression in immune cells is associated with the poor survival of patients with lung cancer with malignant pleural effusion.

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KW - Tumor cell

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