Patient-derived tumor chemosensitization of gkb202, an antrodia cinnamomea mycelium-derived bioactive compound

Tsung Ju Li, Ting Wei Lin, Shih Pei Wu, Hsin Tung Chu, Yu Hsuan Kuo, Jeng Fong Chiou, Long Sheng Lu, Chin Chu Chen

Research output: Contribution to journalArticlepeer-review

Abstract

Oral cancers, hepatocellular carcinoma, and colorectal cancers are the three most common cancers, leading to 18,000 cases of cancer-related mortality in Taiwan per year. To bridge the gap towards clinical translation, we developed a circulating tumor cell (CTC) organoid culture workflow that efficiently expands CTC from patients to test Antrodia Cinnamomea mycelium-derived bioactive compounds. Three ACM-derived bioactive compounds were evaluated for tumor chemosensitization characteristics. Significant and consistent cytotoxic/5-FU sensitizing effects of GKB202 were found on 8 different patient-derived tumors. Acute toxicity profile and hepatic metabolism of GKB202 in rats suggest GKB202 is rapidly cleared by liver and is well tolerated up to the dose of 20 mg/kg. This comprehensive study provides new evidence that liquid fermentation of Antrodia cinnamomea mycelium (ACM) contains bioactive compounds that lead to effective control of CTC, especially when combined with 5-FU. Together, these data suggest ACM-derived GKB202 may be considered for further clinical investigation in the context of 5-FU-based combination therapy.

Original languageEnglish
Article number6018
JournalMolecules
Volume26
Issue number19
DOIs
Publication statusPublished - Oct 1 2021

Keywords

  • 5-FU
  • ACM
  • Antrodia Cinnamomea mycelium
  • Circulating tumor cells
  • Patient-derived organoid

ASJC Scopus subject areas

  • Analytical Chemistry
  • Chemistry (miscellaneous)
  • Molecular Medicine
  • Pharmaceutical Science
  • Drug Discovery
  • Physical and Theoretical Chemistry
  • Organic Chemistry

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