Partial reversion of transformed phenotype of B104 cancer cells by antisense nucleic acids

Yueh Tsern Shaw, Shu Huey Chang, Shean Tai Chiou, Wen Chang Chang, Ming Derg Lai

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We used antisense RNA to inhibit the expression of oncogene neu and investigated the effects of diminished neu expression on the phenotypes of B104 cells containing activated oncogene neu. Antisense MT-neu and pSV-neo plasmids were cotransfected into neuroblastoma B104 cells. Southern analysis showed the integration of antineu DNA into B104 cells. The expression of neu was inhibited up to 90% as quantitated by immunoprecipitation. The growth rate and the potential to differentiate in these transfectants were not affected as compared to the parental cell lines. The ability to grow in soft agar was inhibited more than 90% in these transfectants. Our results indicated that antisense-RNA against a specific oncogene can decrease the tumorigenicity of tumor cells but may not be able to revert it to normal cells completely.

Original languageEnglish
Pages (from-to)27-32
Number of pages6
JournalCancer Letters
Volume69
Issue number1
DOIs
Publication statusPublished - Apr 15 1993
Externally publishedYes

Fingerprint

Nucleic Acids
Phenotype
Oncogenes
Antisense RNA
Neoplasms
Neuroblastoma
Immunoprecipitation
Agar
Plasmids
Cell Line
DNA
Growth

Keywords

  • antisense
  • neu
  • neuroblastoma
  • oncogene

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Partial reversion of transformed phenotype of B104 cancer cells by antisense nucleic acids. / Shaw, Yueh Tsern; Chang, Shu Huey; Chiou, Shean Tai; Chang, Wen Chang; Lai, Ming Derg.

In: Cancer Letters, Vol. 69, No. 1, 15.04.1993, p. 27-32.

Research output: Contribution to journalArticle

Shaw, Yueh Tsern ; Chang, Shu Huey ; Chiou, Shean Tai ; Chang, Wen Chang ; Lai, Ming Derg. / Partial reversion of transformed phenotype of B104 cancer cells by antisense nucleic acids. In: Cancer Letters. 1993 ; Vol. 69, No. 1. pp. 27-32.
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