Parenteral N-3 fatty acids modulate inflammatory and immune response in rats undergoing total gastrectomy

Ming Tsan Lin, Sung Ling Yeh, Chiu Li Yeh, Yu Ni Lai, King Jen Chang, Po Huang Lee, Wei Jao Chen

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

This study investigated the effect of n-3 fatty acid (FA)-containing parenteral nutrition on the circulatory lymphocyte subpopulation, intracellular cytokine and leukocyte adhesion molecule expression, and phagocytic activity in rats undergoing total gastrectomy. Normal rats with internal jugular catheters were assigned to normal control (NC) and two experimental groups and received total parenteral nutrition (TPN). At the same time, a total gastrectomy was performed in the experimental groups, whereas the NC group underwent a sham operation. The TPN solutions were isonitrogenous and identical in nutrient compositions except for differences in fat emulsion contents. The NC and one of the experimental groups received a soybean oil emulsion (SO), and the other experimental group received 50% soybean oil and 50% fish oil emulsion (FO). Half of the rats in each respective group were sacrificed 1 or 3 days after surgery or the sham operation to examine their immune response. The results showed that the FO group had a higher CD4+ proportion and CD4+/ CD8+ ratio than those of the SO and NC groups postoperatively. The phagocytic activity of peritoneal macrophages was higher in the FO group than in the NC group, but no difference was found between the SO and NC groups 3 days after surgery. The intracellular interferon (IFN)-γ distribution in the FO group was higher than that of the SO group on postoperative days. Leukocyte adhesion molecule expressions and peritoneal monocyte chemotactic protein-1 levels were lower in the FO group than in the SO group on postoperative day 3. These results suggest that parenterally infused FO did not result in immunosuppression. In addition, FO administration promotes lymphocyte Th1 cytokine production, enhances peritoneal macrophage phagocytic activity, and reduces leukocyte adhesion molecule expression in rats with total gastrectomy.

Original languageEnglish
Pages (from-to)56-60
Number of pages5
JournalShock
Volume25
Issue number1
DOIs
Publication statusPublished - Jan 2006

Fingerprint

Omega-3 Fatty Acids
Gastrectomy
Emulsions
Fish Oils
Soybean Oil
Cell Adhesion Molecules
Control Groups
Total Parenteral Nutrition
Peritoneal Macrophages
Ambulatory Surgical Procedures
Parenteral Nutrition Solutions
Cytokines
CD4-CD8 Ratio
Chemokine CCL2
Parenteral Nutrition
Lymphocyte Subsets
Immunosuppression
Interferons
Neck
Catheters

Keywords

  • Adhesion molecule
  • Cytokine
  • Gastrectomy
  • Lymphocyte distribution
  • N-3 fatty acids
  • Phagocytic activity

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine
  • Physiology

Cite this

Parenteral N-3 fatty acids modulate inflammatory and immune response in rats undergoing total gastrectomy. / Lin, Ming Tsan; Yeh, Sung Ling; Yeh, Chiu Li; Lai, Yu Ni; Chang, King Jen; Lee, Po Huang; Chen, Wei Jao.

In: Shock, Vol. 25, No. 1, 01.2006, p. 56-60.

Research output: Contribution to journalArticle

Lin, Ming Tsan ; Yeh, Sung Ling ; Yeh, Chiu Li ; Lai, Yu Ni ; Chang, King Jen ; Lee, Po Huang ; Chen, Wei Jao. / Parenteral N-3 fatty acids modulate inflammatory and immune response in rats undergoing total gastrectomy. In: Shock. 2006 ; Vol. 25, No. 1. pp. 56-60.
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abstract = "This study investigated the effect of n-3 fatty acid (FA)-containing parenteral nutrition on the circulatory lymphocyte subpopulation, intracellular cytokine and leukocyte adhesion molecule expression, and phagocytic activity in rats undergoing total gastrectomy. Normal rats with internal jugular catheters were assigned to normal control (NC) and two experimental groups and received total parenteral nutrition (TPN). At the same time, a total gastrectomy was performed in the experimental groups, whereas the NC group underwent a sham operation. The TPN solutions were isonitrogenous and identical in nutrient compositions except for differences in fat emulsion contents. The NC and one of the experimental groups received a soybean oil emulsion (SO), and the other experimental group received 50{\%} soybean oil and 50{\%} fish oil emulsion (FO). Half of the rats in each respective group were sacrificed 1 or 3 days after surgery or the sham operation to examine their immune response. The results showed that the FO group had a higher CD4+ proportion and CD4+/ CD8+ ratio than those of the SO and NC groups postoperatively. The phagocytic activity of peritoneal macrophages was higher in the FO group than in the NC group, but no difference was found between the SO and NC groups 3 days after surgery. The intracellular interferon (IFN)-γ distribution in the FO group was higher than that of the SO group on postoperative days. Leukocyte adhesion molecule expressions and peritoneal monocyte chemotactic protein-1 levels were lower in the FO group than in the SO group on postoperative day 3. These results suggest that parenterally infused FO did not result in immunosuppression. In addition, FO administration promotes lymphocyte Th1 cytokine production, enhances peritoneal macrophage phagocytic activity, and reduces leukocyte adhesion molecule expression in rats with total gastrectomy.",
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AU - Chang, King Jen

AU - Lee, Po Huang

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