Panels of tumor-derived RNA markers in peripheral blood of patients with non-small cell lung cancer: Their dependence on age, gender and clinical stages

Chih Feng Chian, Yi Ting Hwang, Harn Jing Terng, Shih Chun Lee, Tsui Yi Chao, Hung Chang, Ching Liang Ho, Yi Ying Wu, Wann Cherng Perng

Research output: Contribution to journalArticle

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Abstract

Peripheral blood mononuclear cell (PBMC)-derived gene signatures were investigated for their potential use in the early detection of non-small cell lung cancer (NSCLC). In our study, 187 patients with NSCLC and 310 age- and gender-matched controls, and an independent set containing 29 patients for validation were included. Eight significant NSCLC-associated genes were identified, including DUSP6, EIF2S3, GRB2, MDM2, NF1, POLDIP2, RNF4, and WEE1. The logistic model containing these significant markers was able to distinguish subjects with NSCLC from controls with an excellent performance, 80.7% sensitivity, 90.6% specificity, and an area under the receiver operating characteristic curve (AUC) of 0.924. Repeated random subsampling for 100 times was used to validate the performance of classification training models with an average AUC of 0.92. Additional cross-validation using the independent set resulted in the sensitivity 75.86%. Furthermore, six age/gender-dependent genes: CPEB4, EIF2S3, GRB2, MCM4, RNF4, and STAT2 were identified using age and gender stratification approach. STAT2 and WEE1 were explored as stage-dependent using stage-stratified subpopulation. We conclude that these logistic models using different signatures for total and stratified samples are potential complementary tools for assessing the risk of NSCLC.

Original languageEnglish
Pages (from-to)50582-50595
Number of pages14
JournalOncotarget
Volume7
Issue number31
DOIs
Publication statusPublished - 2016

Fingerprint

Non-Small Cell Lung Carcinoma
RNA
Neoplasms
Area Under Curve
Logistic Models
Neoplasm Genes
ROC Curve
Genes
Blood Cells
Sensitivity and Specificity

Keywords

  • Circulating tumor cells
  • Gene expression profiling
  • Non-small cell lung cancer

ASJC Scopus subject areas

  • Oncology

Cite this

Panels of tumor-derived RNA markers in peripheral blood of patients with non-small cell lung cancer : Their dependence on age, gender and clinical stages. / Chian, Chih Feng; Hwang, Yi Ting; Terng, Harn Jing; Lee, Shih Chun; Chao, Tsui Yi; Chang, Hung; Ho, Ching Liang; Wu, Yi Ying; Perng, Wann Cherng.

In: Oncotarget, Vol. 7, No. 31, 2016, p. 50582-50595.

Research output: Contribution to journalArticle

Chian, Chih Feng ; Hwang, Yi Ting ; Terng, Harn Jing ; Lee, Shih Chun ; Chao, Tsui Yi ; Chang, Hung ; Ho, Ching Liang ; Wu, Yi Ying ; Perng, Wann Cherng. / Panels of tumor-derived RNA markers in peripheral blood of patients with non-small cell lung cancer : Their dependence on age, gender and clinical stages. In: Oncotarget. 2016 ; Vol. 7, No. 31. pp. 50582-50595.
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