Paeoniae radix reduces PDGF-stimulated hepatic stellate cell migration

Jong Jen Kuo, Chih Yang Wang, Ting Fang Lee, Yi Tsau Huang, Yun Lian Lin

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Hepatic stellate cells (HSCs) play a key role in the pathogenesis of liver fibrosis. In chronic liver injury, HSCs undergo transdifferentiation to an activated myofibroblastic phenotype and migrate to injured areas in response to chemotactic factors, producing extracellular matrix proteins such as collagen type I to repair the damage as well as overexpression of α-smooth muscle actin (α-SMA). Paeoniae Radix, the root of Paeonia lactiflora Pall, was investigated for PDGF-BB-induced HSC chemotaxis. Rat HSCs and LX-2, a human HSC cell line, were used for the in vitro experiments. Cell migration was analyzed by wound-healing and transwell assays. An ELISA and a Sircol collagen assay kit were used to detect the expressions of α-SMA and of collagen, respectively. Phosphorylations of mitogen-activated protein kinases, including ERK 1/2, p38, and JNK, were evaluated with immunoblotting. Results indicated that PDGF-BB increased migration as well as α-SMA and collagen expression in HSCs. Paeoniae Radix extracts and its active components, paeonol and 1,2,3,4,6-penta-O-galloyl-β-D-glucose (PGG), inhibited PDGF-BB-induced HSC migration and α-SMA and collagen expressions in a concentration-dependent manner. The inhibitory effects were associated with downregulation of PDGF receptor-α, ERK, p38, and JNK activation. Both paeonol and PGG participate in HSC migration, but via differential mechanisms.

Original languageEnglish
Pages (from-to)341-348
Number of pages8
JournalPlanta Medica
Volume78
Issue number4
DOIs
Publication statusPublished - Mar 8 2012
Externally publishedYes

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Keywords

  • 1,2,3,4,6-penta-O-galloyl-β-D-glucose
  • chemotaxis
  • hepatic stellate cells
  • Paeonia lactiflora
  • paeonol
  • PDGF-BB
  • Ranunculaceae

ASJC Scopus subject areas

  • Complementary and alternative medicine
  • Molecular Medicine
  • Organic Chemistry
  • Analytical Chemistry
  • Pharmaceutical Science
  • Pharmacology
  • Drug Discovery

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