P53-dependent downregulation of hTERT protein expression and telomerase activity induces senescence in lung cancer cells as a result of pterostilbene treatment

Rong Jane Chen, Pei Hsuan Wu, Chi Tang Ho, Tzong Der Way, Min Hsiung Pan, Hsiu Min Chen, Yuan Soon Ho, Ying Jan Wang

Research output: Contribution to journalArticle

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Abstract

Cellular senescence is characterized by permanent cell cycle arrest, triggered by a variety of stresses, such as telomerase inhibition, and it is recognized as a tumor-suppressor mechanism. In recent years, telomerase has become an important therapeutic target in several cancers; inhibition of telomerase can induce senescence via the DNA damage response (DDR). Pterostilbene (PT), a dimethyl ether analog of resveratrol, possesses a variety of biological functions, including anticancer effects; however, the molecular mechanisms underlying these effects are not fully understood. In this study, we investigated the possible mechanisms of PT-induced senescence through telomerase inhibition in human non-small cell lung cancer cells and delineated the role of p53 in senescence. The results indicated that PT-induced senescence is characterized by a flattened morphology, positive staining for senescence-associated-β galactosidase activity, and the formation of senescence-associated heterochromatic foci. Telomerase activity and protein expression was significantly decreased in H460 (p53 wild type) cells compared with H1299 (p53 null) cells and p53 knockdown H460 cells (H460-p53-). A more detailed mechanistic study revealed that PT-induced senescence partially occurred via a p53-dependent mechanism, triggering inhibition of telomerase activity and protein expression, and leading to the DDR, S phase arrest and, finally, cellular senescence. This study is the first to explore the novel anticancer mechanism of PT senescence induction via the inhibition of telomerase in lung cancer cells.

Original languageEnglish
Pages (from-to)e2985
JournalCell death & disease
Volume8
Issue number8
DOIs
Publication statusPublished - Aug 10 2017

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Telomerase
Lung Neoplasms
Down-Regulation
Proteins
Cell Aging
DNA Damage
Galactosidases
Null Lymphocytes
pterostilbene
Cell Cycle Checkpoints
S Phase
Non-Small Cell Lung Carcinoma
Neoplasms
Staining and Labeling

ASJC Scopus subject areas

  • Immunology
  • Cellular and Molecular Neuroscience
  • Cell Biology
  • Cancer Research

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P53-dependent downregulation of hTERT protein expression and telomerase activity induces senescence in lung cancer cells as a result of pterostilbene treatment. / Chen, Rong Jane; Wu, Pei Hsuan; Ho, Chi Tang; Way, Tzong Der; Pan, Min Hsiung; Chen, Hsiu Min; Ho, Yuan Soon; Wang, Ying Jan.

In: Cell death & disease, Vol. 8, No. 8, 10.08.2017, p. e2985.

Research output: Contribution to journalArticle

Chen, Rong Jane ; Wu, Pei Hsuan ; Ho, Chi Tang ; Way, Tzong Der ; Pan, Min Hsiung ; Chen, Hsiu Min ; Ho, Yuan Soon ; Wang, Ying Jan. / P53-dependent downregulation of hTERT protein expression and telomerase activity induces senescence in lung cancer cells as a result of pterostilbene treatment. In: Cell death & disease. 2017 ; Vol. 8, No. 8. pp. e2985.
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