P16 methylation is an early event in cervical carcinogenesis

Lee Wen Huang, Hun Shan Pan, Yu Hung Lin, Kok Min Seow, Heng Ju Chen, Jiann Loung Hwang

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Background: Aberrant gene promoter methylation is a critical event in tumorigenesis. The aim of this study was to explore the promoter hypermethylation of p16 and DAPK1 during the progression of cervical precancerous lesions. Methods: A series of 98 cervical neoplasms (72 cervical intraepithelial neoplasia and 26 cervical carcinomas) were evaluated. The promoter methylation status of p16 and DAPK1 was assessed from cervical scrapings by methylation-specific polymerase chain reaction. Results: For p16, the frequency of promoter hypermethylation showed an increasing trend from normal to dysplastic to invasive squamous cancer specimens, and this increase reached statistical significance (P <0.0001). However, there was no significant difference in the promoter methylation state of DAPK1 with regard to the various grades of cervical lesions (P = 0.077). Specifically, methylation of p16 was a frequent event in the cervical carcinoma samples, and these figures were statistically significant compared with the normal and cervical intraepithelial neoplasia I cases (P = 0.015 and P = 0.021, respectively). Conclusions: These results imply that promoter hypermethylation of p16 occurs at an early stage of cervical neoplastic progression. This early event may play an initiating role in the malignant transformation of low-grade dysplasia into high-grade dysplasia and invasive carcinoma. We suggest that aberrant promoter methylation of p16 may serve as a useful biomarker during the follow-up of low-grade dysplasia.

Original languageEnglish
Pages (from-to)452-456
Number of pages5
JournalInternational Journal of Gynecological Cancer
Volume21
Issue number3
DOIs
Publication statusPublished - Apr 2011

Fingerprint

Methylation
Carcinogenesis
Cervical Intraepithelial Neoplasia
Carcinoma
Uterine Cervical Neoplasms
Biomarkers
Polymerase Chain Reaction
Genes
Neoplasms

Keywords

  • Cervical carcinoma
  • Cervical intraepithelial neoplasia
  • Methylation
  • p16

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Oncology
  • Medicine(all)

Cite this

Huang, L. W., Pan, H. S., Lin, Y. H., Seow, K. M., Chen, H. J., & Hwang, J. L. (2011). P16 methylation is an early event in cervical carcinogenesis. International Journal of Gynecological Cancer, 21(3), 452-456. https://doi.org/10.1097/IGC.0b013e31821091ea

P16 methylation is an early event in cervical carcinogenesis. / Huang, Lee Wen; Pan, Hun Shan; Lin, Yu Hung; Seow, Kok Min; Chen, Heng Ju; Hwang, Jiann Loung.

In: International Journal of Gynecological Cancer, Vol. 21, No. 3, 04.2011, p. 452-456.

Research output: Contribution to journalArticle

Huang, LW, Pan, HS, Lin, YH, Seow, KM, Chen, HJ & Hwang, JL 2011, 'P16 methylation is an early event in cervical carcinogenesis', International Journal of Gynecological Cancer, vol. 21, no. 3, pp. 452-456. https://doi.org/10.1097/IGC.0b013e31821091ea
Huang, Lee Wen ; Pan, Hun Shan ; Lin, Yu Hung ; Seow, Kok Min ; Chen, Heng Ju ; Hwang, Jiann Loung. / P16 methylation is an early event in cervical carcinogenesis. In: International Journal of Gynecological Cancer. 2011 ; Vol. 21, No. 3. pp. 452-456.
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