Oxidative stress induces ADAM9 protein expression in human prostate cancer cells

Shian Ying Sung, Hiroyuki Kubo, Katsumi Shigemura, Rebecca S. Arnold, Sanjay Logani, Ruoxiang Wang, Hiroyuki Konaka, Masayuki Nakagawa, Spiro Mousses, Mahul Amin, Cynthia Anderson, Peter Johnstone, John A. Petros, Fray F. Marshall, Haiyen E. Zhau, Leland W K Chung

Research output: Contribution to journalArticle

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Abstract

The ADAM (a disintegrin and metalloprotease) family is a group of transmembrane proteins containing cell adhesive and proteolytic functional domains. Microarray analysis detected elevated ADAM9 during the transition of human LNCaP prostate cancer cells from an androgen-dependent to an androgen-independent and metastatic state. Using a prostate tissue array (N = 200), the levels of ADAM9 protein expression were also elevated in malignant as compared with benign prostate tissues. ADAM9 protein expression was found in 43% of benign glands with light staining and 87% of malignant glands with increasing intensity of staining. We found that ADAM9 mRNA and protein expressions were elevated on exposure of human prostate cancer cells to stress conditions such as cell crowding, hypoxia, and hydrogen peroxide. We uncovered an ADAM9-like protein, which is predominantly induced together with the ADAM9 protein by a brief exposure of prostate cancer cells to hydrogen peroxide. Induction of ADAM9 protein in LNCaP or C4-2 cells can be completely abrogated by the administration of an antioxidant, ebselen, or genetic transfer of a hydrogen peroxide degradative enzyme, catalase, suggesting that reactive oxygen species (ROS) are a common mediator. The induction of ADAM9 by stress can be inhibited by both actinomycin D and cycloheximide through increased gene transcription and protein synthesis. In conclusion, intracellular ROS and/or hydrogen peroxide, generated by cell stress, regulate ADAM9 expression. ADAM9 could be responsible for supporting prostate cancer cell survival and progression. By decreasing ADAM9 expression, we observed apoptotic cell death in prostate cancer cells.

Original languageEnglish
Pages (from-to)9519-9526
Number of pages8
JournalCancer Research
Volume66
Issue number19
DOIs
Publication statusPublished - Oct 1 2006
Externally publishedYes

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Prostatic Neoplasms
Oxidative Stress
Hydrogen Peroxide
Proteins
Androgens
Prostate
Reactive Oxygen Species
Staining and Labeling
Disintegrins
Cell Hypoxia
Crowding
Metalloproteases
Dactinomycin
Cycloheximide
Microarray Analysis
Adhesives
Catalase
Cell Survival
Cell Death
Antioxidants

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Sung, S. Y., Kubo, H., Shigemura, K., Arnold, R. S., Logani, S., Wang, R., ... Chung, L. W. K. (2006). Oxidative stress induces ADAM9 protein expression in human prostate cancer cells. Cancer Research, 66(19), 9519-9526. https://doi.org/10.1158/0008-5472.CAN-05-4375

Oxidative stress induces ADAM9 protein expression in human prostate cancer cells. / Sung, Shian Ying; Kubo, Hiroyuki; Shigemura, Katsumi; Arnold, Rebecca S.; Logani, Sanjay; Wang, Ruoxiang; Konaka, Hiroyuki; Nakagawa, Masayuki; Mousses, Spiro; Amin, Mahul; Anderson, Cynthia; Johnstone, Peter; Petros, John A.; Marshall, Fray F.; Zhau, Haiyen E.; Chung, Leland W K.

In: Cancer Research, Vol. 66, No. 19, 01.10.2006, p. 9519-9526.

Research output: Contribution to journalArticle

Sung, SY, Kubo, H, Shigemura, K, Arnold, RS, Logani, S, Wang, R, Konaka, H, Nakagawa, M, Mousses, S, Amin, M, Anderson, C, Johnstone, P, Petros, JA, Marshall, FF, Zhau, HE & Chung, LWK 2006, 'Oxidative stress induces ADAM9 protein expression in human prostate cancer cells', Cancer Research, vol. 66, no. 19, pp. 9519-9526. https://doi.org/10.1158/0008-5472.CAN-05-4375
Sung, Shian Ying ; Kubo, Hiroyuki ; Shigemura, Katsumi ; Arnold, Rebecca S. ; Logani, Sanjay ; Wang, Ruoxiang ; Konaka, Hiroyuki ; Nakagawa, Masayuki ; Mousses, Spiro ; Amin, Mahul ; Anderson, Cynthia ; Johnstone, Peter ; Petros, John A. ; Marshall, Fray F. ; Zhau, Haiyen E. ; Chung, Leland W K. / Oxidative stress induces ADAM9 protein expression in human prostate cancer cells. In: Cancer Research. 2006 ; Vol. 66, No. 19. pp. 9519-9526.
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AU - Logani, Sanjay

AU - Wang, Ruoxiang

AU - Konaka, Hiroyuki

AU - Nakagawa, Masayuki

AU - Mousses, Spiro

AU - Amin, Mahul

AU - Anderson, Cynthia

AU - Johnstone, Peter

AU - Petros, John A.

AU - Marshall, Fray F.

AU - Zhau, Haiyen E.

AU - Chung, Leland W K

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