Oxidative stress facilitates IFN-γ-induced mimic extracellular trap cell death in A549 lung epithelial cancer cells

Chiou-Feng Lin, Chia-Ling Chen, Shun Yi Chien, Po Chun Tseng, Yu Chih Wang, Tsung Ting Tsai

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We previously demonstrated that IFN-γ induces an autophagy-regulated mimic extracellular trap cell death (ETosis) in A549 human lung cancer cells. Regarding reactive oxygen species (ROS) are involved in ETosis, this study investigated the role of oxidative stress. After IFN-γ stimulation, a necrosis-like cell death mimic ETosis occurred accompanied by the inhibition of cell growth, aberrant nuclear staining, and nucleosome release. ROS were generated in a time-dependent manner with an increase in NADPH oxidase component protein expression. STAT1-mediated IFN regulatory factor-1 activation was essential for upregulating ROS production. By genetically silencing p47phox, IFN-γ-induced ROS and mimic ETosis were significantly attenuated. This mechanistic study indicated that ROS may mediate DNA damage followed by histone H3 citrullination. Furthermore, ROS promoted IFN-γ-induced mimic ETosis in cooperation with autophagy. These findings further demonstrate that ROS regulates IFN-γ-induced mimic ETosis in lung epithelial malignancy.

Original languageEnglish
Article numbere0162157
JournalPLoS One
Volume11
Issue number8
DOIs
Publication statusPublished - Aug 1 2016

Fingerprint

Oxidative stress
Cell death
cell death
reactive oxygen species
Reactive Oxygen Species
Lung Neoplasms
Oxidative Stress
epithelial cells
Cell Death
oxidative stress
traps
Epithelial Cells
lungs
Cells
autophagy
Autophagy
nucleosomes
Nucleosomes
NADPH Oxidase
Cell growth

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Oxidative stress facilitates IFN-γ-induced mimic extracellular trap cell death in A549 lung epithelial cancer cells. / Lin, Chiou-Feng; Chen, Chia-Ling; Chien, Shun Yi; Tseng, Po Chun; Wang, Yu Chih; Tsai, Tsung Ting.

In: PLoS One, Vol. 11, No. 8, e0162157, 01.08.2016.

Research output: Contribution to journalArticle

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