Oxidative stress enhances Axl-mediated cell migration through an Akt1/Rac1-dependent mechanism

Jhy Shrian Huang, Chun Yu Cho, Chih Chen Hong, Ming De Yan, Mao-Chih Hsieh, Jong Ding Lay, Gi Ming Lai, Ann Lii Cheng, Shuang En Chuang

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Persistent oxidative stress is common in cancer cells because of abnormal generation of reactive oxygen species (ROS) and has been associated with malignant phenotypes, such as chemotherapy resistance and metastasis. Both overexpression of Axl and abnormal ROS elevation have been linked to cell transformation and increased cell migration. However, the relationship between Axl and ROS in malignant cell migration has not been previously evaluated. Using an in vitro human lung cancer model, we examined the redox state of lung adenocarcinoma cell lines of low metastatic (CL1-0) and high metastatic (CL1-5) potentials. Here we report that Axl activation elicits ROS accumulation through the oxidase-coupled small GTPase Rac1. We also observed that oxidative stress could activate Axl phosphorylation to synergistically enhance cell migration. Further, Axl signaling activated by H2O2 treatment results in enhancement of cell migration via a PI3K/Akt-dependent pathway. The kinase activity of Axl is required for the Axl-mediated cell migration and prolongs the half-life of phospho-Akt under oxidative stress. Finally, downregulation of Akt1, but not Akt2, by RNAi in Axl-overexpressing cells inhibits the amount of activated Rac1 and the ability to migrate induced by H2O2 treatment. Together, these results show that a novel Axl-signaling cascade induced by H2O2 treatment triggers cell migration through the PI3K/Akt1/Rac1 pathway. Elucidation of redox regulation in Axl-related malignant migration may provide new molecular insights into the mechanisms underlying tumor progression.

Original languageEnglish
Pages (from-to)1246-1256
Number of pages11
JournalFree Radical Biology and Medicine
Volume65
DOIs
Publication statusPublished - 2013

Fingerprint

Oxidative stress
Cell Movement
Reactive Oxygen Species
Oxidative Stress
Phosphatidylinositol 3-Kinases
Cells
Phosphorylation
Oxidation-Reduction
Chemotherapy
Monomeric GTP-Binding Proteins
Tumors
Oxidoreductases
Phosphotransferases
Chemical activation
RNA Interference
Half-Life
Lung Neoplasms
Neoplasms
Therapeutics
Down-Regulation

Keywords

  • Akt1
  • Axl
  • Free radicals
  • NSCLC
  • Oxidative stress
  • Rac1
  • ROS

ASJC Scopus subject areas

  • Biochemistry
  • Physiology (medical)

Cite this

Oxidative stress enhances Axl-mediated cell migration through an Akt1/Rac1-dependent mechanism. / Huang, Jhy Shrian; Cho, Chun Yu; Hong, Chih Chen; Yan, Ming De; Hsieh, Mao-Chih; Lay, Jong Ding; Lai, Gi Ming; Cheng, Ann Lii; Chuang, Shuang En.

In: Free Radical Biology and Medicine, Vol. 65, 2013, p. 1246-1256.

Research output: Contribution to journalArticle

Huang, Jhy Shrian ; Cho, Chun Yu ; Hong, Chih Chen ; Yan, Ming De ; Hsieh, Mao-Chih ; Lay, Jong Ding ; Lai, Gi Ming ; Cheng, Ann Lii ; Chuang, Shuang En. / Oxidative stress enhances Axl-mediated cell migration through an Akt1/Rac1-dependent mechanism. In: Free Radical Biology and Medicine. 2013 ; Vol. 65. pp. 1246-1256.
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