Oxidative DNA damage in human peripheral leukocytes induced by massive aerobic exercise

Kelvin Tsai, Tai Ger Hsu, Kuang Ming Hsu, Hu Cheng, Tsung Yun Liu, Chen Fu Hsu, Chi Woon Kong

Research output: Contribution to journalArticle

171 Citations (Scopus)

Abstract

Reactive oxygen species produced during vigorous exercise may permeate into cell nuclei and induce oxidative DNA damage, but the supporting evidence is still lacking. By using a 42 km marathon race as a model of massive aerobic exercise, we demonstrated a significant degree of unrepaired DNA base oxidation in peripheral immunocompetent cells, despite a concurrent increase in the urinary excretion of 8-hydroxy-2′-deoxyguanosine. Single cell gel electrophoresis with the incorporation of lesion-specific endonucleases further revealed that oxidized pyrimidines (endonuclease III-sensitive sites) contributed to most of the postexercise nucleotide oxidation. The oxidative DNA damage correlated significantly with plasma levels of creatinine kinase and lipid peroxidation metabolites, and lasted for more than 1 week following the race. This phenomenon may be one of the mechanisms behind the immune dysfunctions after exhaustive exercise.

Original languageEnglish
Pages (from-to)1465-1472
Number of pages8
JournalFree Radical Biology and Medicine
Volume31
Issue number11
DOIs
Publication statusPublished - Dec 1 2001
Externally publishedYes

Fingerprint

DNA Damage
Leukocytes
Endonucleases
Exercise
DNA
Oxidation
Pyrimidines
Comet Assay
Metabolites
Electrophoresis
Cell Nucleus
Lipid Peroxidation
Reactive Oxygen Species
Creatinine
Phosphotransferases
Nucleotides
Gels
Cells
Lipids
Plasmas

Keywords

  • Comet assay
  • Free radicals
  • Humans
  • Oxidants
  • Running

ASJC Scopus subject areas

  • Medicine(all)
  • Toxicology
  • Clinical Biochemistry

Cite this

Oxidative DNA damage in human peripheral leukocytes induced by massive aerobic exercise. / Tsai, Kelvin; Hsu, Tai Ger; Hsu, Kuang Ming; Cheng, Hu; Liu, Tsung Yun; Hsu, Chen Fu; Kong, Chi Woon.

In: Free Radical Biology and Medicine, Vol. 31, No. 11, 01.12.2001, p. 1465-1472.

Research output: Contribution to journalArticle

Tsai, Kelvin ; Hsu, Tai Ger ; Hsu, Kuang Ming ; Cheng, Hu ; Liu, Tsung Yun ; Hsu, Chen Fu ; Kong, Chi Woon. / Oxidative DNA damage in human peripheral leukocytes induced by massive aerobic exercise. In: Free Radical Biology and Medicine. 2001 ; Vol. 31, No. 11. pp. 1465-1472.
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