Oxidative DNA damage in human peripheral leukocytes induced by massive aerobic exercise

Kelvin Tsai, Tai Ger Hsu, Kuang Ming Hsu, Hu Cheng, Tsung Yun Liu, Chen Fu Hsu, Chi Woon Kong

Research output: Contribution to journalArticlepeer-review

192 Citations (Scopus)


Reactive oxygen species produced during vigorous exercise may permeate into cell nuclei and induce oxidative DNA damage, but the supporting evidence is still lacking. By using a 42 km marathon race as a model of massive aerobic exercise, we demonstrated a significant degree of unrepaired DNA base oxidation in peripheral immunocompetent cells, despite a concurrent increase in the urinary excretion of 8-hydroxy-2′-deoxyguanosine. Single cell gel electrophoresis with the incorporation of lesion-specific endonucleases further revealed that oxidized pyrimidines (endonuclease III-sensitive sites) contributed to most of the postexercise nucleotide oxidation. The oxidative DNA damage correlated significantly with plasma levels of creatinine kinase and lipid peroxidation metabolites, and lasted for more than 1 week following the race. This phenomenon may be one of the mechanisms behind the immune dysfunctions after exhaustive exercise.

Original languageEnglish
Pages (from-to)1465-1472
Number of pages8
JournalFree Radical Biology and Medicine
Issue number11
Publication statusPublished - Dec 1 2001
Externally publishedYes


  • Comet assay
  • Free radicals
  • Humans
  • Oxidants
  • Running

ASJC Scopus subject areas

  • Medicine(all)
  • Toxicology
  • Clinical Biochemistry


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