Overexpression of protein kinase Cα mRNA may be an independent prognostic marker for gastric carcinoma

Kai Yuan Lin, Chia Lang Fang, Yih Huei Uen, Chun Chao Chang, Horng Yuan Lou, Ching Ruey Hsieh, Cheng Tiong, Shiann Pan, Sheng-Hsuan Chen

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background and Objectives: The variability of the prognosis of gastric carcinoma drives extensive researches for novel prognostic markers. The aims of this study were to correlate the expression of protein kinase Cα (PKCα) mRNA with clinicopathological parameters and to evaluate the significant value of PKCα in gastric carcinoma prognosis. Methods: PKCα mRNA levels were analyzed in tumor/non-tumor pairs of gastric tissues from surgical specimens of 41 patients with gastric carcinoma employing quantitative real-time polymerase chain reaction. Expression of PKCα in gastric carcinoma was also examined using immunohistochemistry. Results: PKCα mRNA expression was significantly upregulated in gastric carcinoma (P = 0.007). Overexpression of PKCα mRNA was correlated with distant metastasis (P = 0.040). Patients with high PKCα mRNA expression had a significantly poorer overall survival compared with patients with low PKCα mRNA expression (P = 0.0113). The uni-variate Cox regression analysis showed that high PKCα mRNA expression (P = 0.0363) and depth of invasion (P = 0.0443) were two significant prognostic markers for gastric carcinoma. In backward stepwise multivariate analysis, PKCα mRNA overexpression was also proved to be an independent prognostic marker for gastric carcinoma (P = 0.0275). Conclusions: Our results suggest that overexpression of PKCα mRNA has correlation with distant metastasis and may be an independent prognostic marker for gastric carcinoma.

Original languageEnglish
Pages (from-to)538-543
Number of pages6
JournalJournal of Surgical Oncology
Volume97
Issue number6
DOIs
Publication statusPublished - May 1 2008

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Protein Kinase C
Stomach
Carcinoma
Messenger RNA
Neoplasm Metastasis
Real-Time Polymerase Chain Reaction
Multivariate Analysis
Immunohistochemistry
Regression Analysis
Survival

Keywords

  • Distant metastasis
  • Gastric carcinoma
  • Prognosis
  • Protein kinase Cα

ASJC Scopus subject areas

  • Surgery
  • Oncology

Cite this

Overexpression of protein kinase Cα mRNA may be an independent prognostic marker for gastric carcinoma. / Lin, Kai Yuan; Fang, Chia Lang; Uen, Yih Huei; Chang, Chun Chao; Lou, Horng Yuan; Hsieh, Ching Ruey; Tiong, Cheng; Pan, Shiann; Chen, Sheng-Hsuan.

In: Journal of Surgical Oncology, Vol. 97, No. 6, 01.05.2008, p. 538-543.

Research output: Contribution to journalArticle

Lin, Kai Yuan ; Fang, Chia Lang ; Uen, Yih Huei ; Chang, Chun Chao ; Lou, Horng Yuan ; Hsieh, Ching Ruey ; Tiong, Cheng ; Pan, Shiann ; Chen, Sheng-Hsuan. / Overexpression of protein kinase Cα mRNA may be an independent prognostic marker for gastric carcinoma. In: Journal of Surgical Oncology. 2008 ; Vol. 97, No. 6. pp. 538-543.
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abstract = "Background and Objectives: The variability of the prognosis of gastric carcinoma drives extensive researches for novel prognostic markers. The aims of this study were to correlate the expression of protein kinase Cα (PKCα) mRNA with clinicopathological parameters and to evaluate the significant value of PKCα in gastric carcinoma prognosis. Methods: PKCα mRNA levels were analyzed in tumor/non-tumor pairs of gastric tissues from surgical specimens of 41 patients with gastric carcinoma employing quantitative real-time polymerase chain reaction. Expression of PKCα in gastric carcinoma was also examined using immunohistochemistry. Results: PKCα mRNA expression was significantly upregulated in gastric carcinoma (P = 0.007). Overexpression of PKCα mRNA was correlated with distant metastasis (P = 0.040). Patients with high PKCα mRNA expression had a significantly poorer overall survival compared with patients with low PKCα mRNA expression (P = 0.0113). The uni-variate Cox regression analysis showed that high PKCα mRNA expression (P = 0.0363) and depth of invasion (P = 0.0443) were two significant prognostic markers for gastric carcinoma. In backward stepwise multivariate analysis, PKCα mRNA overexpression was also proved to be an independent prognostic marker for gastric carcinoma (P = 0.0275). Conclusions: Our results suggest that overexpression of PKCα mRNA has correlation with distant metastasis and may be an independent prognostic marker for gastric carcinoma.",
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