Abstract
Aim: To describe the significant over-expression of fibroblast growth factor receptor 3 (FGFR3), which is a signal transduction and cell proliferation related gene in hepatocellular carcinoma (HCC). Methods: Following DNA microarray, Northern blot and quantitative real-time PCR were employed to confirm FGFR3 expression difference in HCC tissues and surrounding non-neoplastic liver tissue. FGFR3 expression levels were further determined by immunohistochemical study in 43 cases of HCC. Results: Northern blot results showed the significant over-expression of FGFR3 in HCC tissues, which was consistent with that from DNA microarray. Quantitative real-time PCR demonstrated that the mean ratio of FGFR3 mRNA to glyceraldehyde-3-phosphate dehydrogenase (GADPH) mRNA in HCC tissue was 0.250, whereas the ratio in non-neoplastic liver tissue was 0.014. Statistical analyses of 43 cases of HCC revealed that HCC scored higher than the matched non-neoplastic liver tissues. Examination of clinicopathological features revealed a strong correlation of over-expression of FGFR3 with poor tumor differentiation and high nuclear grade. Conclusion: Over-expression of FGFR3 may play an important role in liver carcinogenesis. FGFR3 may be an ideal candidate as a molecular marker in the diagnosis of HCC and a potential therapeutic target.
| Original language | English |
|---|---|
| Pages (from-to) | 5266-5272 |
| Number of pages | 7 |
| Journal | World Journal of Gastroenterology |
| Volume | 11 |
| Issue number | 34 |
| DOIs | |
| Publication status | Published - Sep 14 2005 |
| Externally published | Yes |
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Keywords
- Fibroblast growth factor receptor 3
- Human hepatocellular carcinoma
- Microarray
ASJC Scopus subject areas
- Gastroenterology
Cite this
Over-expression of fibroblast growth factor receptor 3 in human hepatocellular carcinoma. / Qiu, Wei Hua; Zhou, Bing Sen; Chu, Peiguo G.; Chen, Wen Gang; Chung, Christopher; Shih, Jennifer; Hwu, Paul; Yeh, Christopher; Lopez, Richard; Yen, Yun.
In: World Journal of Gastroenterology, Vol. 11, No. 34, 14.09.2005, p. 5266-5272.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Over-expression of fibroblast growth factor receptor 3 in human hepatocellular carcinoma
AU - Qiu, Wei Hua
AU - Zhou, Bing Sen
AU - Chu, Peiguo G.
AU - Chen, Wen Gang
AU - Chung, Christopher
AU - Shih, Jennifer
AU - Hwu, Paul
AU - Yeh, Christopher
AU - Lopez, Richard
AU - Yen, Yun
PY - 2005/9/14
Y1 - 2005/9/14
N2 - Aim: To describe the significant over-expression of fibroblast growth factor receptor 3 (FGFR3), which is a signal transduction and cell proliferation related gene in hepatocellular carcinoma (HCC). Methods: Following DNA microarray, Northern blot and quantitative real-time PCR were employed to confirm FGFR3 expression difference in HCC tissues and surrounding non-neoplastic liver tissue. FGFR3 expression levels were further determined by immunohistochemical study in 43 cases of HCC. Results: Northern blot results showed the significant over-expression of FGFR3 in HCC tissues, which was consistent with that from DNA microarray. Quantitative real-time PCR demonstrated that the mean ratio of FGFR3 mRNA to glyceraldehyde-3-phosphate dehydrogenase (GADPH) mRNA in HCC tissue was 0.250, whereas the ratio in non-neoplastic liver tissue was 0.014. Statistical analyses of 43 cases of HCC revealed that HCC scored higher than the matched non-neoplastic liver tissues. Examination of clinicopathological features revealed a strong correlation of over-expression of FGFR3 with poor tumor differentiation and high nuclear grade. Conclusion: Over-expression of FGFR3 may play an important role in liver carcinogenesis. FGFR3 may be an ideal candidate as a molecular marker in the diagnosis of HCC and a potential therapeutic target.
AB - Aim: To describe the significant over-expression of fibroblast growth factor receptor 3 (FGFR3), which is a signal transduction and cell proliferation related gene in hepatocellular carcinoma (HCC). Methods: Following DNA microarray, Northern blot and quantitative real-time PCR were employed to confirm FGFR3 expression difference in HCC tissues and surrounding non-neoplastic liver tissue. FGFR3 expression levels were further determined by immunohistochemical study in 43 cases of HCC. Results: Northern blot results showed the significant over-expression of FGFR3 in HCC tissues, which was consistent with that from DNA microarray. Quantitative real-time PCR demonstrated that the mean ratio of FGFR3 mRNA to glyceraldehyde-3-phosphate dehydrogenase (GADPH) mRNA in HCC tissue was 0.250, whereas the ratio in non-neoplastic liver tissue was 0.014. Statistical analyses of 43 cases of HCC revealed that HCC scored higher than the matched non-neoplastic liver tissues. Examination of clinicopathological features revealed a strong correlation of over-expression of FGFR3 with poor tumor differentiation and high nuclear grade. Conclusion: Over-expression of FGFR3 may play an important role in liver carcinogenesis. FGFR3 may be an ideal candidate as a molecular marker in the diagnosis of HCC and a potential therapeutic target.
KW - Fibroblast growth factor receptor 3
KW - Human hepatocellular carcinoma
KW - Microarray
UR - http://www.scopus.com/inward/record.url?scp=26244433313&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=26244433313&partnerID=8YFLogxK
U2 - 10.3748/wjg.v11.i34.5266
DO - 10.3748/wjg.v11.i34.5266
M3 - Article
VL - 11
SP - 5266
EP - 5272
JO - World Journal of Gastroenterology
JF - World Journal of Gastroenterology
SN - 1007-9327
IS - 34
ER -