Over-expression of fibroblast growth factor receptor 3 in human hepatocellular carcinoma

Wei Hua Qiu, Bing Sen Zhou, Peiguo G. Chu, Wen Gang Chen, Christopher Chung, Jennifer Shih, Paul Hwu, Christopher Yeh, Richard Lopez, Yun Yen

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Aim: To describe the significant over-expression of fibroblast growth factor receptor 3 (FGFR3), which is a signal transduction and cell proliferation related gene in hepatocellular carcinoma (HCC). Methods: Following DNA microarray, Northern blot and quantitative real-time PCR were employed to confirm FGFR3 expression difference in HCC tissues and surrounding non-neoplastic liver tissue. FGFR3 expression levels were further determined by immunohistochemical study in 43 cases of HCC. Results: Northern blot results showed the significant over-expression of FGFR3 in HCC tissues, which was consistent with that from DNA microarray. Quantitative real-time PCR demonstrated that the mean ratio of FGFR3 mRNA to glyceraldehyde-3-phosphate dehydrogenase (GADPH) mRNA in HCC tissue was 0.250, whereas the ratio in non-neoplastic liver tissue was 0.014. Statistical analyses of 43 cases of HCC revealed that HCC scored higher than the matched non-neoplastic liver tissues. Examination of clinicopathological features revealed a strong correlation of over-expression of FGFR3 with poor tumor differentiation and high nuclear grade. Conclusion: Over-expression of FGFR3 may play an important role in liver carcinogenesis. FGFR3 may be an ideal candidate as a molecular marker in the diagnosis of HCC and a potential therapeutic target.

Original languageEnglish
Pages (from-to)5266-5272
Number of pages7
JournalWorld Journal of Gastroenterology
Volume11
Issue number34
DOIs
Publication statusPublished - Sep 14 2005
Externally publishedYes

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Receptor, Fibroblast Growth Factor, Type 3
Hepatocellular Carcinoma
Liver
Oligonucleotide Array Sequence Analysis
Northern Blotting
Real-Time Polymerase Chain Reaction
Messenger RNA
Glyceraldehyde-3-Phosphate Dehydrogenases
Signal Transduction
Carcinogenesis
Cell Proliferation

Keywords

  • Fibroblast growth factor receptor 3
  • Human hepatocellular carcinoma
  • Microarray

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Over-expression of fibroblast growth factor receptor 3 in human hepatocellular carcinoma. / Qiu, Wei Hua; Zhou, Bing Sen; Chu, Peiguo G.; Chen, Wen Gang; Chung, Christopher; Shih, Jennifer; Hwu, Paul; Yeh, Christopher; Lopez, Richard; Yen, Yun.

In: World Journal of Gastroenterology, Vol. 11, No. 34, 14.09.2005, p. 5266-5272.

Research output: Contribution to journalArticle

Qiu, WH, Zhou, BS, Chu, PG, Chen, WG, Chung, C, Shih, J, Hwu, P, Yeh, C, Lopez, R & Yen, Y 2005, 'Over-expression of fibroblast growth factor receptor 3 in human hepatocellular carcinoma', World Journal of Gastroenterology, vol. 11, no. 34, pp. 5266-5272. https://doi.org/10.3748/wjg.v11.i34.5266
Qiu, Wei Hua ; Zhou, Bing Sen ; Chu, Peiguo G. ; Chen, Wen Gang ; Chung, Christopher ; Shih, Jennifer ; Hwu, Paul ; Yeh, Christopher ; Lopez, Richard ; Yen, Yun. / Over-expression of fibroblast growth factor receptor 3 in human hepatocellular carcinoma. In: World Journal of Gastroenterology. 2005 ; Vol. 11, No. 34. pp. 5266-5272.
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