Outcome of Intracytoplasmic Injection of Sperm Obtained by Testicular Sperm Extraction from 14 Azoospermic Men Suffering from 47,XXY Non-mosaic Klinefelter's Syndrome

Chun Ming Chiang, Chuan Ju Lin, Liang Ming Lee, Shih Min Chen

Research output: Contribution to journalArticle

Abstract

Objective: The purposes of this study were to evaluate the potential for testicular sperm extraction (TESE) from azoospermic patients with non-mosaic Klinefelter's syndrome and to determine the outcome of intracytoplasmic sperm injection (ICSI) using the extracted testicular sperm sample. Materials and Methods: Fourteen couples suffering from primary infertility in which the male partner had the azoospermic non-mosaic 47,XXY karyotype (Klinefelter's syndrome) participated in this study. All of the women underwent controlled ovarian hyperstimulation. Open testis biopsies were conducted 1 day prior to or on the day of oocyte retrieval. Motile sperm, extracted from the biopsied tissues in a wet preparation, were used for ICSI. The outcome of ICSI was evaluated from the fertilization rate, embryo-cleavage rate, clinical pregnancy rate, and chromosomal status of resultant fetuses or delivered babies. Results: Sperm retrieval was successful in eight of 14 patients (sperm retrieval rate, 57%). In total, 118 mature oocytes were injected with extracted motile spermatozoa. This resulted in the production of 70 fertilized oocytes (fertilization rate, 59%) and 67 embryos (cleavage rate, 96%). Among the eight women who underwent embryo transfer, six achieved clinical pregnancies (clinical pregnancy rate, 75%). The outcome of these pregnancies included one blighted ovum and the birth of four male and five female healthy babies. The live delivery rate was 62.5%. All of these babies were chromosomally and physiologically normal. Conclusion: This study demonstrates that azoospermic patients suffering from non-mosaic Klinefelter's syndrome can father their own genetic offspring when the TESE procedure is combined with ICSI and embryo transfer techniques.

Original languageEnglish
Pages (from-to)88-96
Number of pages9
JournalTaiwanese Journal of Obstetrics and Gynecology
Volume43
Issue number2
DOIs
Publication statusPublished - Jun 2004

Fingerprint

Klinefelter Syndrome
Intracytoplasmic Sperm Injections
Spermatozoa
Sperm Retrieval
Embryo Transfer
Pregnancy Rate
Fertilization
Oocytes
Embryonic Structures
Oocyte Retrieval
Pregnancy Outcome
Karyotype
Fathers
Infertility
Ovum
Testis
Fetus
Parturition
Biopsy
Pregnancy

Keywords

  • 47,XXY karyotype
  • azoospermia
  • intracytoplasmic sperm injection
  • Klinefelter's syndrome
  • testicular sperm extraction

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

Cite this

Outcome of Intracytoplasmic Injection of Sperm Obtained by Testicular Sperm Extraction from 14 Azoospermic Men Suffering from 47,XXY Non-mosaic Klinefelter's Syndrome. / Chiang, Chun Ming; Lin, Chuan Ju; Lee, Liang Ming; Chen, Shih Min.

In: Taiwanese Journal of Obstetrics and Gynecology, Vol. 43, No. 2, 06.2004, p. 88-96.

Research output: Contribution to journalArticle

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title = "Outcome of Intracytoplasmic Injection of Sperm Obtained by Testicular Sperm Extraction from 14 Azoospermic Men Suffering from 47,XXY Non-mosaic Klinefelter's Syndrome",
abstract = "Objective: The purposes of this study were to evaluate the potential for testicular sperm extraction (TESE) from azoospermic patients with non-mosaic Klinefelter's syndrome and to determine the outcome of intracytoplasmic sperm injection (ICSI) using the extracted testicular sperm sample. Materials and Methods: Fourteen couples suffering from primary infertility in which the male partner had the azoospermic non-mosaic 47,XXY karyotype (Klinefelter's syndrome) participated in this study. All of the women underwent controlled ovarian hyperstimulation. Open testis biopsies were conducted 1 day prior to or on the day of oocyte retrieval. Motile sperm, extracted from the biopsied tissues in a wet preparation, were used for ICSI. The outcome of ICSI was evaluated from the fertilization rate, embryo-cleavage rate, clinical pregnancy rate, and chromosomal status of resultant fetuses or delivered babies. Results: Sperm retrieval was successful in eight of 14 patients (sperm retrieval rate, 57{\%}). In total, 118 mature oocytes were injected with extracted motile spermatozoa. This resulted in the production of 70 fertilized oocytes (fertilization rate, 59{\%}) and 67 embryos (cleavage rate, 96{\%}). Among the eight women who underwent embryo transfer, six achieved clinical pregnancies (clinical pregnancy rate, 75{\%}). The outcome of these pregnancies included one blighted ovum and the birth of four male and five female healthy babies. The live delivery rate was 62.5{\%}. All of these babies were chromosomally and physiologically normal. Conclusion: This study demonstrates that azoospermic patients suffering from non-mosaic Klinefelter's syndrome can father their own genetic offspring when the TESE procedure is combined with ICSI and embryo transfer techniques.",
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AU - Chen, Shih Min

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N2 - Objective: The purposes of this study were to evaluate the potential for testicular sperm extraction (TESE) from azoospermic patients with non-mosaic Klinefelter's syndrome and to determine the outcome of intracytoplasmic sperm injection (ICSI) using the extracted testicular sperm sample. Materials and Methods: Fourteen couples suffering from primary infertility in which the male partner had the azoospermic non-mosaic 47,XXY karyotype (Klinefelter's syndrome) participated in this study. All of the women underwent controlled ovarian hyperstimulation. Open testis biopsies were conducted 1 day prior to or on the day of oocyte retrieval. Motile sperm, extracted from the biopsied tissues in a wet preparation, were used for ICSI. The outcome of ICSI was evaluated from the fertilization rate, embryo-cleavage rate, clinical pregnancy rate, and chromosomal status of resultant fetuses or delivered babies. Results: Sperm retrieval was successful in eight of 14 patients (sperm retrieval rate, 57%). In total, 118 mature oocytes were injected with extracted motile spermatozoa. This resulted in the production of 70 fertilized oocytes (fertilization rate, 59%) and 67 embryos (cleavage rate, 96%). Among the eight women who underwent embryo transfer, six achieved clinical pregnancies (clinical pregnancy rate, 75%). The outcome of these pregnancies included one blighted ovum and the birth of four male and five female healthy babies. The live delivery rate was 62.5%. All of these babies were chromosomally and physiologically normal. Conclusion: This study demonstrates that azoospermic patients suffering from non-mosaic Klinefelter's syndrome can father their own genetic offspring when the TESE procedure is combined with ICSI and embryo transfer techniques.

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