Osteoporosis recovery by antrodia camphorata alcohol extracts through bone regeneration in SAMP8 Mice

Hen Yu Liu, Chiung Fang Huang, Chun Hao Li, Ching Yu Tsai, Wei Hong Chen, Hong Jian Wei, Ming Fu Wang, Yueh Hsiung Kuo, Mei Leng Cheong, Win Ping Deng

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Abstract

Antrodia camphorata has previously demonstrated the efficacy in treating cancer and anti-inflammation. In this study, we are the first to evaluate Antrodia camphorata alcohol extract (ACAE) for osteoporosis recovery in vitro with preosteoblast cells (MC3T3-E1) and in vivo with an osteoporosis mouse model established in our previous studies, ovariectomized senescence accelerated mice (OVX-SAMP8). Our results demonstrated that ACAE treatment was slightly cytotoxic to preosteoblast at 25 μg/mL, by which the osteogenic gene expression (RUNX2, OPN, and OCN) was significantly upregulated with an increased ratio of OPG to RANKL, indicating maintenance of the bone matrix through inhibition of osteoclastic pathway. Additionally, evaluation by Alizarin Red S staining showed increased mineralization in ACAE-treated preosteoblasts. For in vivo study, our results indicated that ACAE inhibits bone loss and significantly increases percentage bone volume, trabecular bone number, and bone mineral density in OVX-SAMP8 mice treated with ACAE. Collectively, in vitro and in vivo results showed that ACAE could promote osteogenesis and prevent bone loss and should be considered an evidence-based complementary and alternative medicine for osteoporosis therapy through the maintenance of bone health.

Original languageEnglish
Article number2617868
JournalEvidence-based Complementary and Alternative Medicine
Volume2016
DOIs
Publication statusPublished - 2016

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ASJC Scopus subject areas

  • Complementary and alternative medicine

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