Osteopontin is involved in migration of eosinophils in asthma

A. Takahashi, M. Kurokawa, S. Konno, K. Ito, S. Kon, S. Ashino, T. Nishimura, T. Uede, N. Hizawa, S. K. Huang, M. Nishimura

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Background Osteopontin (OPN) is an extracellular matrix protein with a wide range of functions, and is involved in various inflammatory diseases. However, the role of OPN in eosinophilic airway inflammation is unclear. Objective To elucidate the role of OPN in eosinophilic airway inflammation. Methods OPN protein levels in induced sputum from asthmatic patients and healthy controls were measured. Eosinophil migration assays were performed in the presence or absence of OPN, a blocking antibody (Ab) recognizing its integrin-binding domain (2K1) and an anti-integrin α4 Ab (P1H4). In the mouse asthma model, the levels of eosinophilia were examined in bronchoalveolar lavage fluids (BALFs) from ovalbumin (OVA)-sensitized and -challenged mice with or without administration of an Ab (M5) corresponding to human 2K1. Results Levels of OPN in induced sputum were significantly higher in asthmatic patients when compared with healthy controls. In addition, levels of OPN were correlated with the percentage of sputum eosinophils. OPN induced significant migration of human eosinophils and this effect was inhibited by 2K1 and P1H4. M5 significantly attenuated OVA-induced eosinophilia in BALFs. Conclusion These results indicate that OPN plays a role in the migration of eosinophils into the airways and may be involved in the pathogenesis of asthma.

Original languageEnglish
Pages (from-to)1152-1159
Number of pages8
JournalClinical and Experimental Allergy
Volume39
Issue number8
DOIs
Publication statusPublished - Aug 1 2009
Externally publishedYes

Fingerprint

Osteopontin
Eosinophils
Asthma
Sputum
Ovalbumin
Bronchoalveolar Lavage Fluid
Eosinophilia
Integrins
Inflammation
Blocking Antibodies
Antibodies
Extracellular Matrix Proteins

Keywords

  • Asthma
  • Eosinophil
  • Migration
  • Osteopontin

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Takahashi, A., Kurokawa, M., Konno, S., Ito, K., Kon, S., Ashino, S., ... Nishimura, M. (2009). Osteopontin is involved in migration of eosinophils in asthma. Clinical and Experimental Allergy, 39(8), 1152-1159. https://doi.org/10.1111/j.1365-2222.2009.03249.x

Osteopontin is involved in migration of eosinophils in asthma. / Takahashi, A.; Kurokawa, M.; Konno, S.; Ito, K.; Kon, S.; Ashino, S.; Nishimura, T.; Uede, T.; Hizawa, N.; Huang, S. K.; Nishimura, M.

In: Clinical and Experimental Allergy, Vol. 39, No. 8, 01.08.2009, p. 1152-1159.

Research output: Contribution to journalArticle

Takahashi, A, Kurokawa, M, Konno, S, Ito, K, Kon, S, Ashino, S, Nishimura, T, Uede, T, Hizawa, N, Huang, SK & Nishimura, M 2009, 'Osteopontin is involved in migration of eosinophils in asthma', Clinical and Experimental Allergy, vol. 39, no. 8, pp. 1152-1159. https://doi.org/10.1111/j.1365-2222.2009.03249.x
Takahashi A, Kurokawa M, Konno S, Ito K, Kon S, Ashino S et al. Osteopontin is involved in migration of eosinophils in asthma. Clinical and Experimental Allergy. 2009 Aug 1;39(8):1152-1159. https://doi.org/10.1111/j.1365-2222.2009.03249.x
Takahashi, A. ; Kurokawa, M. ; Konno, S. ; Ito, K. ; Kon, S. ; Ashino, S. ; Nishimura, T. ; Uede, T. ; Hizawa, N. ; Huang, S. K. ; Nishimura, M. / Osteopontin is involved in migration of eosinophils in asthma. In: Clinical and Experimental Allergy. 2009 ; Vol. 39, No. 8. pp. 1152-1159.
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abstract = "Background Osteopontin (OPN) is an extracellular matrix protein with a wide range of functions, and is involved in various inflammatory diseases. However, the role of OPN in eosinophilic airway inflammation is unclear. Objective To elucidate the role of OPN in eosinophilic airway inflammation. Methods OPN protein levels in induced sputum from asthmatic patients and healthy controls were measured. Eosinophil migration assays were performed in the presence or absence of OPN, a blocking antibody (Ab) recognizing its integrin-binding domain (2K1) and an anti-integrin α4 Ab (P1H4). In the mouse asthma model, the levels of eosinophilia were examined in bronchoalveolar lavage fluids (BALFs) from ovalbumin (OVA)-sensitized and -challenged mice with or without administration of an Ab (M5) corresponding to human 2K1. Results Levels of OPN in induced sputum were significantly higher in asthmatic patients when compared with healthy controls. In addition, levels of OPN were correlated with the percentage of sputum eosinophils. OPN induced significant migration of human eosinophils and this effect was inhibited by 2K1 and P1H4. M5 significantly attenuated OVA-induced eosinophilia in BALFs. Conclusion These results indicate that OPN plays a role in the migration of eosinophils into the airways and may be involved in the pathogenesis of asthma.",
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