Osteopontin as an injury marker expressing in epithelial hyperplasia lesions helpful in prognosis of focal segmental glomerulosclerosis

Hao Ai Shui, Shuk Man Ka, Shun Min Yang, Yuh Feng Lin, Yi Fen Lo, Ann Chen

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Focal segmental glomerulosclerosis (FSGS) is characterized by typical sclerosis but also shows other non-sclerotic lesions that provide prognostic informations. The glomerular epithelial hyperplasia lesion (EPHL) that develops earlier than the sclerotic lesions is a key determinant of progression of FSGS. However, the relationship among EPHL, glomeular sclerosis, and macrophage infiltration in FSGS is unclear, and the EPHL-associated markers helpful for prognosis of FSGS have still not been completely identified. Here, we performed clinicopathologic, immunochemical, and molecular analyses to examine whether osteopontin (OPN), a macrophage chemokine, is an injury marker of EPHLs correlating with glomerular sclerosis and macrophage mobilization. First, the FSGS model was induced in Balb/c mice by a single injection of adriamycin, and consecutive sclerosis changes were evaluated. In parallel, we used reverse transcription-polymerase chain reaction and Western blot analyses to determine levels of OPN in isolated glomeruli and urine, respectively. Immunohistochemistry was applied to assess the OPN expression in EPHLs and macrophage infiltration around the glomeruli. Our results showed that, within glomeruli, OPN expressed restrictedly within EPHL; the OPN mRNA and protein of glomeruli increased on day 11, correlating well with the early EPHL, and following sclerosis and macrophage infiltration. In addition, immunohistochemistry (IHC) staining of OPN greatly highlighted early glomerular EPHLs, helping microscopic identification of EPHLs. We propose that the OPN expression in EPHLs could contribute to the progression of FSGS by recruiting macrophage toward the compromised glomeruli. Detection of OPN in glomeruli and urine could be helpful in prognosis of FSGS.

Original languageEnglish
Pages (from-to)216-222
Number of pages7
JournalTranslational Research
Volume150
Issue number4
DOIs
Publication statusPublished - Oct 2007
Externally publishedYes

Fingerprint

Focal Segmental Glomerulosclerosis
Osteopontin
Hyperplasia
Macrophages
Sclerosis
Wounds and Injuries
Infiltration
Immunohistochemistry
Urine
Polymerase chain reaction
Transcription
Chemokines
Doxorubicin
Reverse Transcription
Western Blotting
Staining and Labeling
Polymerase Chain Reaction
Messenger RNA
Injections

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, medical
  • Public Health, Environmental and Occupational Health

Cite this

Osteopontin as an injury marker expressing in epithelial hyperplasia lesions helpful in prognosis of focal segmental glomerulosclerosis. / Shui, Hao Ai; Ka, Shuk Man; Yang, Shun Min; Lin, Yuh Feng; Lo, Yi Fen; Chen, Ann.

In: Translational Research, Vol. 150, No. 4, 10.2007, p. 216-222.

Research output: Contribution to journalArticle

Shui, Hao Ai ; Ka, Shuk Man ; Yang, Shun Min ; Lin, Yuh Feng ; Lo, Yi Fen ; Chen, Ann. / Osteopontin as an injury marker expressing in epithelial hyperplasia lesions helpful in prognosis of focal segmental glomerulosclerosis. In: Translational Research. 2007 ; Vol. 150, No. 4. pp. 216-222.
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