Abstract

Suicide gene therapy using the herpes simplex virus thymidine kinase (HSV-tk) gene, combined with the prodrug ganciclovir (GCV) medication, is a promising approach for the treatment of malignant tumors, including prostate cancer. The success of this therapeutic strategy requires tissue- or tumor-specific gene expression and efficient gene delivery. In this chapter, we describe the experimental protocols of key methodologies, including promoter construction, reporter assay, adenoviral vector construction and preparation, HSV-tk enzymatic assay and cytotoxicity assay to evaluate the specificity and efficacy of osteonectin promoter-mediated HSV-tk/GCV suicide gene therapy of prostate cancer.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages27-42
Number of pages16
DOIs
Publication statusPublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1895
ISSN (Print)1064-3745

Keywords

  • Adenoviral vector
  • HSV-tk enzymatic assay
  • Osteonectin promoter construction
  • Prostate cancer
  • Suicide gene therapy

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Hsiao, W. C., Sung, S. Y., Chung, L. W. K., & Hsieh, C. L. (2019). Osteonectin promoter-mediated suicide gene therapy of prostate cancer. In Methods in Molecular Biology (pp. 27-42). (Methods in Molecular Biology; Vol. 1895). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-8922-5_3