Osteoinduction and Osteogenesis of a Novel Bone Morphogenetic Protein-Conjugated Poly-(D, L-Lactide-Co-Glycolide), Hydroxyapatite, and Collagen Composite Bone Substitute

Chih-Hwa Chen, 劉 席瑋(Hsia-Wei Liu), 蔡 清霖(Ching-Lin Tsai), 陳 文哲(Wen-Jer Chen), 施 俊雄(Chun-Hsiung Shih), 薛 敬和(Ging-Ho Hsiue)

Research output: Contribution to journalArticle

Abstract

We developed a bone substitute consisting of 3-dimensional poly-(D, L-lactide-co-glycolide) and hydroxyapatite scaffolds coated with type I collagen (PLGA/HA/collagen). Recombinant human bone morphogenetic protein-2 (rhBMP-2), which stimulated bone regeneration, was immobilized on this scaffold to deliver growth factor. Osteoinduction and osteogenesis were assessed with MG-63 human osteoblast-like cells in vitro and in animals in vivo. At 7 days, seeded MG-63 cells were equally distributed throughout the surface, with cellular pseudopodia attachments. The composite showed high cellular activity with osteoblastic proliferation and a dense, bonelike layer. Assay with 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide showed significant cellular proliferation and viability. Alkaline phosphatase assay showed significantly enhanced osteogenic differentiation. In vivo, the filling material showed evidence of resorption and new bone formation. The bone void was completely replaced by bone ingrowths at 12 weeks. The osteoconductive PLGA/HA scaffold with the bioactive collagen coating and rhBMP-2 tethering stimulated osteoinduction and osteogenesis. Immobilization of rhBMP-2 on the scaffold provided a multifunctional and biomimetic delivery vehicle for growth factor to enhance cell adherence, proliferation, and differentiation. This novel composite scaffold induced osteoinduction and osteogenesis may be useful as a synthetic substitute for bone regeneration and for future orthopaedic tissue engineering.
Original languageEnglish
Pages (from-to)131-141
Number of pages11
JournalJournal of Orthopedic Surgery Taiwan
Volume22
Issue number3
Publication statusPublished - 2005

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Bone Substitutes
Bone Morphogenetic Proteins
Durapatite
Osteogenesis
Collagen
Bone Regeneration
Intercellular Signaling Peptides and Proteins
Cell Proliferation
Bone and Bones
Biomimetics
Pseudopodia
Tissue Engineering
Collagen Type I
Osteoblasts
Immobilization
Orthopedics
Alkaline Phosphatase
Cell Differentiation
polylactic acid-polyglycolic acid copolymer
recombinant human bone morphogenetic protein-2

Keywords

  • poly-(D, L-lactide-co-glycolide)
  • hydroxyapatite
  • collagen
  • rhBMP-2
  • tissue engineering

Cite this

Osteoinduction and Osteogenesis of a Novel Bone Morphogenetic Protein-Conjugated Poly-(D, L-Lactide-Co-Glycolide), Hydroxyapatite, and Collagen Composite Bone Substitute. / Chen, Chih-Hwa; 劉席瑋(Hsia-Wei Liu); 蔡清霖(Ching-Lin Tsai); 陳文哲(Wen-Jer Chen); 施俊雄(Chun-Hsiung Shih); 薛敬和(Ging-Ho Hsiue).

In: Journal of Orthopedic Surgery Taiwan, Vol. 22, No. 3, 2005, p. 131-141.

Research output: Contribution to journalArticle

Chen, C-H, 劉席瑋(Hsia-WeiL, 蔡清霖(Ching-LinT, 陳文哲(Wen-JerC, 施俊雄(Chun-HsiungS & 薛敬和(Ging-HoH 2005, 'Osteoinduction and Osteogenesis of a Novel Bone Morphogenetic Protein-Conjugated Poly-(D, L-Lactide-Co-Glycolide), Hydroxyapatite, and Collagen Composite Bone Substitute', Journal of Orthopedic Surgery Taiwan, vol. 22, no. 3, pp. 131-141.
Chen, Chih-Hwa ; 劉席瑋(Hsia-Wei Liu) ; 蔡清霖(Ching-Lin Tsai) ; 陳文哲(Wen-Jer Chen) ; 施俊雄(Chun-Hsiung Shih) ; 薛敬和(Ging-Ho Hsiue). / Osteoinduction and Osteogenesis of a Novel Bone Morphogenetic Protein-Conjugated Poly-(D, L-Lactide-Co-Glycolide), Hydroxyapatite, and Collagen Composite Bone Substitute. In: Journal of Orthopedic Surgery Taiwan. 2005 ; Vol. 22, No. 3. pp. 131-141.
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abstract = "We developed a bone substitute consisting of 3-dimensional poly-(D, L-lactide-co-glycolide) and hydroxyapatite scaffolds coated with type I collagen (PLGA/HA/collagen). Recombinant human bone morphogenetic protein-2 (rhBMP-2), which stimulated bone regeneration, was immobilized on this scaffold to deliver growth factor. Osteoinduction and osteogenesis were assessed with MG-63 human osteoblast-like cells in vitro and in animals in vivo. At 7 days, seeded MG-63 cells were equally distributed throughout the surface, with cellular pseudopodia attachments. The composite showed high cellular activity with osteoblastic proliferation and a dense, bonelike layer. Assay with 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide showed significant cellular proliferation and viability. Alkaline phosphatase assay showed significantly enhanced osteogenic differentiation. In vivo, the filling material showed evidence of resorption and new bone formation. The bone void was completely replaced by bone ingrowths at 12 weeks. The osteoconductive PLGA/HA scaffold with the bioactive collagen coating and rhBMP-2 tethering stimulated osteoinduction and osteogenesis. Immobilization of rhBMP-2 on the scaffold provided a multifunctional and biomimetic delivery vehicle for growth factor to enhance cell adherence, proliferation, and differentiation. This novel composite scaffold induced osteoinduction and osteogenesis may be useful as a synthetic substitute for bone regeneration and for future orthopaedic tissue engineering.",
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AU - 劉, 席瑋(Hsia-Wei Liu)

AU - 蔡, 清霖(Ching-Lin Tsai)

AU - 陳, 文哲(Wen-Jer Chen)

AU - 施, 俊雄(Chun-Hsiung Shih)

AU - 薛, 敬和(Ging-Ho Hsiue)

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N2 - We developed a bone substitute consisting of 3-dimensional poly-(D, L-lactide-co-glycolide) and hydroxyapatite scaffolds coated with type I collagen (PLGA/HA/collagen). Recombinant human bone morphogenetic protein-2 (rhBMP-2), which stimulated bone regeneration, was immobilized on this scaffold to deliver growth factor. Osteoinduction and osteogenesis were assessed with MG-63 human osteoblast-like cells in vitro and in animals in vivo. At 7 days, seeded MG-63 cells were equally distributed throughout the surface, with cellular pseudopodia attachments. The composite showed high cellular activity with osteoblastic proliferation and a dense, bonelike layer. Assay with 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide showed significant cellular proliferation and viability. Alkaline phosphatase assay showed significantly enhanced osteogenic differentiation. In vivo, the filling material showed evidence of resorption and new bone formation. The bone void was completely replaced by bone ingrowths at 12 weeks. The osteoconductive PLGA/HA scaffold with the bioactive collagen coating and rhBMP-2 tethering stimulated osteoinduction and osteogenesis. Immobilization of rhBMP-2 on the scaffold provided a multifunctional and biomimetic delivery vehicle for growth factor to enhance cell adherence, proliferation, and differentiation. This novel composite scaffold induced osteoinduction and osteogenesis may be useful as a synthetic substitute for bone regeneration and for future orthopaedic tissue engineering.

AB - We developed a bone substitute consisting of 3-dimensional poly-(D, L-lactide-co-glycolide) and hydroxyapatite scaffolds coated with type I collagen (PLGA/HA/collagen). Recombinant human bone morphogenetic protein-2 (rhBMP-2), which stimulated bone regeneration, was immobilized on this scaffold to deliver growth factor. Osteoinduction and osteogenesis were assessed with MG-63 human osteoblast-like cells in vitro and in animals in vivo. At 7 days, seeded MG-63 cells were equally distributed throughout the surface, with cellular pseudopodia attachments. The composite showed high cellular activity with osteoblastic proliferation and a dense, bonelike layer. Assay with 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide showed significant cellular proliferation and viability. Alkaline phosphatase assay showed significantly enhanced osteogenic differentiation. In vivo, the filling material showed evidence of resorption and new bone formation. The bone void was completely replaced by bone ingrowths at 12 weeks. The osteoconductive PLGA/HA scaffold with the bioactive collagen coating and rhBMP-2 tethering stimulated osteoinduction and osteogenesis. Immobilization of rhBMP-2 on the scaffold provided a multifunctional and biomimetic delivery vehicle for growth factor to enhance cell adherence, proliferation, and differentiation. This novel composite scaffold induced osteoinduction and osteogenesis may be useful as a synthetic substitute for bone regeneration and for future orthopaedic tissue engineering.

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