Osteogenesis of human adipose-derived stem cells on hydroxyapatite-mineralized poly(lactic acid) nanofiber sheets

Fu Chen Kung, Chi Chang Lin, Wen Fu T Lai

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Electrospun fiber sheets with various orientations (random, partially aligned, and aligned) and smooth and roughened casted membranes were prepared. Hydroxyapatite (HA) crystals were in situ formed on these material surfaces via immersion in 10× simulated body fluid solution. The size and morphology of the resulting fibers were examined using scanning electron microscopy. The average diameter of the fibers ranged from 225±25 to 1050 ± 150 nm depending on the electrospinning parameters. Biological experiment results show that human adipose-derived stem cells exhibit different adhesion and osteogenic differentiation on the three types of fiber. The cell proliferation and osteogenic differentiation were best on the aligned fibers. Similar results were found for phosphorylated focal adhesion kinase expression. Electrospun poly(lactic acid) aligned fibers mineralized with HA crystals provide a good environment for cell growth and osteogenic differentiation and thus have great potential in the tissue engineering field.

Original languageEnglish
Pages (from-to)578-588
Number of pages11
JournalMaterials Science and Engineering C
Volume45
DOIs
Publication statusPublished - Dec 1 2014

Fingerprint

osteogenesis
lactic acid
stem cells
Durapatite
Lactic acid
Nanofibers
Stem cells
Hydroxyapatite
fibers
Fibers
adhesion
Adhesion
Focal Adhesion Protein-Tyrosine Kinases
Crystals
body fluids
tissue engineering
Body fluids
Bioelectric potentials
Cell proliferation
Electrospinning

Keywords

  • Bone tissue
  • Electrospun
  • Human adipose-derived stem cells
  • Mineralization
  • Simulated body fluid

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering
  • Mechanics of Materials

Cite this

Osteogenesis of human adipose-derived stem cells on hydroxyapatite-mineralized poly(lactic acid) nanofiber sheets. / Kung, Fu Chen; Lin, Chi Chang; Lai, Wen Fu T.

In: Materials Science and Engineering C, Vol. 45, 01.12.2014, p. 578-588.

Research output: Contribution to journalArticle

@article{53f09c8fd30340b792cacc07c6072b6f,
title = "Osteogenesis of human adipose-derived stem cells on hydroxyapatite-mineralized poly(lactic acid) nanofiber sheets",
abstract = "Electrospun fiber sheets with various orientations (random, partially aligned, and aligned) and smooth and roughened casted membranes were prepared. Hydroxyapatite (HA) crystals were in situ formed on these material surfaces via immersion in 10× simulated body fluid solution. The size and morphology of the resulting fibers were examined using scanning electron microscopy. The average diameter of the fibers ranged from 225±25 to 1050 ± 150 nm depending on the electrospinning parameters. Biological experiment results show that human adipose-derived stem cells exhibit different adhesion and osteogenic differentiation on the three types of fiber. The cell proliferation and osteogenic differentiation were best on the aligned fibers. Similar results were found for phosphorylated focal adhesion kinase expression. Electrospun poly(lactic acid) aligned fibers mineralized with HA crystals provide a good environment for cell growth and osteogenic differentiation and thus have great potential in the tissue engineering field.",
keywords = "Bone tissue, Electrospun, Human adipose-derived stem cells, Mineralization, Simulated body fluid",
author = "Kung, {Fu Chen} and Lin, {Chi Chang} and Lai, {Wen Fu T}",
year = "2014",
month = "12",
day = "1",
doi = "10.1016/j.msec.2014.10.005",
language = "English",
volume = "45",
pages = "578--588",
journal = "Materials Science and Engineering C",
issn = "0928-4931",
publisher = "Elsevier BV",

}

TY - JOUR

T1 - Osteogenesis of human adipose-derived stem cells on hydroxyapatite-mineralized poly(lactic acid) nanofiber sheets

AU - Kung, Fu Chen

AU - Lin, Chi Chang

AU - Lai, Wen Fu T

PY - 2014/12/1

Y1 - 2014/12/1

N2 - Electrospun fiber sheets with various orientations (random, partially aligned, and aligned) and smooth and roughened casted membranes were prepared. Hydroxyapatite (HA) crystals were in situ formed on these material surfaces via immersion in 10× simulated body fluid solution. The size and morphology of the resulting fibers were examined using scanning electron microscopy. The average diameter of the fibers ranged from 225±25 to 1050 ± 150 nm depending on the electrospinning parameters. Biological experiment results show that human adipose-derived stem cells exhibit different adhesion and osteogenic differentiation on the three types of fiber. The cell proliferation and osteogenic differentiation were best on the aligned fibers. Similar results were found for phosphorylated focal adhesion kinase expression. Electrospun poly(lactic acid) aligned fibers mineralized with HA crystals provide a good environment for cell growth and osteogenic differentiation and thus have great potential in the tissue engineering field.

AB - Electrospun fiber sheets with various orientations (random, partially aligned, and aligned) and smooth and roughened casted membranes were prepared. Hydroxyapatite (HA) crystals were in situ formed on these material surfaces via immersion in 10× simulated body fluid solution. The size and morphology of the resulting fibers were examined using scanning electron microscopy. The average diameter of the fibers ranged from 225±25 to 1050 ± 150 nm depending on the electrospinning parameters. Biological experiment results show that human adipose-derived stem cells exhibit different adhesion and osteogenic differentiation on the three types of fiber. The cell proliferation and osteogenic differentiation were best on the aligned fibers. Similar results were found for phosphorylated focal adhesion kinase expression. Electrospun poly(lactic acid) aligned fibers mineralized with HA crystals provide a good environment for cell growth and osteogenic differentiation and thus have great potential in the tissue engineering field.

KW - Bone tissue

KW - Electrospun

KW - Human adipose-derived stem cells

KW - Mineralization

KW - Simulated body fluid

UR - http://www.scopus.com/inward/record.url?scp=84908280225&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84908280225&partnerID=8YFLogxK

U2 - 10.1016/j.msec.2014.10.005

DO - 10.1016/j.msec.2014.10.005

M3 - Article

AN - SCOPUS:84908280225

VL - 45

SP - 578

EP - 588

JO - Materials Science and Engineering C

JF - Materials Science and Engineering C

SN - 0928-4931

ER -