Orientation and position of avian leukosis virus DNA relative to the cellular oncogene c-myc in B-lymphoma tumors of highly susceptible 15I5 x 72 chickens

Y. K T Fung, L. B. Crittenden, H. J. Kung

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

We previously reported our characterizations of the B-lymphoma tumors induced in a highly susceptible line of chickens (15I5 x 72) by the avian leukosis virus RAV-1 (Proc. Natl. Acad. Sci. 78:3418-3422, 1981). We demonstrated that in >90% of the tumors, the RAV-1 provirus is integrated near a cellular oncogene, c-myc. In the present study, we devised a simple approach, relying on SalI digestion, for further defining the locations and orientations of the proviruses with respect to the c-myc gene. We report here that in the great majority of cases the provirus is situated upstream from and in the same transcriptional direction as the c-myc gene - a configuration compatible with the promoter-insertion model proposed by Hayward et al. (Nature [London] 290:475-480, 1981).

Original languageEnglish
Pages (from-to)742-746
Number of pages5
JournalJournal of Virology
Volume44
Issue number2
Publication statusPublished - Dec 1 1982
Externally publishedYes

Fingerprint

Avian Leukosis Virus
Avian leukosis virus
proviruses
Proviruses
myc Genes
oncogenes
lymphoma
Chickens
Lymphoma
chickens
neoplasms
DNA
Neoplasms
Digestion
genes
digestion
promoter regions

ASJC Scopus subject areas

  • Immunology

Cite this

Orientation and position of avian leukosis virus DNA relative to the cellular oncogene c-myc in B-lymphoma tumors of highly susceptible 15I5 x 72 chickens. / Fung, Y. K T; Crittenden, L. B.; Kung, H. J.

In: Journal of Virology, Vol. 44, No. 2, 01.12.1982, p. 742-746.

Research output: Contribution to journalArticle

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