Orally administered mycelial culture of Phellinus linteus exhibits antitumor effects in hepatoma cell-bearing mice

Hui Yu Huang, Shih Yung Chieh, Tim K. Tso, Ting Yi Chien, Hsin Tang Lin, Ying Chieh Tsai

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Aim of the study: The aim of this study was to evaluate the anticancer effect of a mycelial culture from Phellinus linteus PL-7 (MCPL-7) and to elucidate its potential mechanism in vivo. Materials and methods: SCID CB-17 mice received a transplant of Hep3B cells followed by daily MCPL-7 administrations for 8 weeks. Following tumor implantation, groups C-E were subcutaneously administered 50 mg/kg, 100 mg/kg, or 250 mg/kg MCPL-7 powder per day, respectively, for 8 weeks. Groups A and B received saline solution subcutaneously for 8 weeks. Results: MCPL-7 administration induced a significant reduction in tumor size and was associated with a significant increase in T cell numbers; IL-12, IFN-γ and TNF-α secretion; NK cell activity; and phagocytic ability. Therefore, increased numbers of CD4+cells could have been caused by greater numbers of dendritic cells and macrophages in the spleen. Furthermore, the activation of dendritic cells and macrophages resulted in increased IL-12 secretion, which could upregulate NK cell activation. The increased secretion of IL-12, IFN-γ, and TNF-α enhanced the activity and phagocytic ability of NK cells. Thus, MCPL-7 may provide a potential therapeutic approach for both immunomodulatory and antitumor effects.

Original languageEnglish
Pages (from-to)460-466
Number of pages7
JournalJournal of Ethnopharmacology
Volume133
Issue number2
DOIs
Publication statusPublished - Jan 27 2011
Externally publishedYes

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Interleukin-12
Natural Killer Cells
Hepatocellular Carcinoma
Dendritic Cells
Cell Count
Macrophages
Sodium Chloride
Powders
Neoplasms
Up-Regulation
Spleen
T-Lymphocytes
Transplants
Therapeutics

Keywords

  • Antitumor
  • Immunomodulation
  • Phellinus linteus

ASJC Scopus subject areas

  • Pharmacology
  • Drug Discovery

Cite this

Orally administered mycelial culture of Phellinus linteus exhibits antitumor effects in hepatoma cell-bearing mice. / Huang, Hui Yu; Chieh, Shih Yung; Tso, Tim K.; Chien, Ting Yi; Lin, Hsin Tang; Tsai, Ying Chieh.

In: Journal of Ethnopharmacology, Vol. 133, No. 2, 27.01.2011, p. 460-466.

Research output: Contribution to journalArticle

Huang, Hui Yu ; Chieh, Shih Yung ; Tso, Tim K. ; Chien, Ting Yi ; Lin, Hsin Tang ; Tsai, Ying Chieh. / Orally administered mycelial culture of Phellinus linteus exhibits antitumor effects in hepatoma cell-bearing mice. In: Journal of Ethnopharmacology. 2011 ; Vol. 133, No. 2. pp. 460-466.
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