Oral toxicity evaluation of kefir-isolated Lactobacillus kefiranofaciens M1 in Sprague-Dawley rats

E. E. Owaga, M. J. Chen, W. Y. Chen, C. W. Chen, R. H. Hsieh

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Lactobacilli kefiranofaciens M1 has shown novel immunomodulation and anti-allergy probiotic attributes in cell and animal models. An acute oral toxicity assessment of L. kefiranofaciens M1 was evaluated in Sprague-Dawley rats. The rats were randomly assigned to four groups (12 rats/sex/group): the low dose group was orally gavaged with L. kefiranofaciens M1 at 3.0×108cfu/kg bw while the medium dose and high dose groups received 9.0×109cfu/kg bw and 1.8×1010cfu/kg bw, respectively, for 28days. The control group received phosphate buffer saline. The body weights were measured weekly while blood samples were collected for haematology and serum biochemistry tests. Histopathology of the organs (heart, liver, kidney, adrenal glands, spleen, ovary, testis), and urinalysis were conducted on study termination. The body weight gain of the L. kefiranofaciens M1 and control groups were comparable during the administration period. Overall, L. kefiranofaciens M1 did not induce adverse effects on haematology, serum biochemistry, and urinalysis parameters. Gross and microscopic histopathology of the organs revealed no toxicity effect of L. kefiranofaciens M1. In conclusion, 1.8×1010cfu/kg bw of L. kefiranofaciens M1 was considered as the no-observed-adverse-effect-level (NOAEL), which was the highest dose tested in the present study.

Original languageEnglish
Pages (from-to)157-162
Number of pages6
JournalFood and Chemical Toxicology
Volume70
DOIs
Publication statusPublished - 2014

Fingerprint

Lactobacillus kefiri
drug toxicity
Urinalysis
Lactobacillus
Hematology
Biochemistry
Sprague Dawley Rats
Toxicity
Rats
Body Weight
No-Observed-Adverse-Effect Level
Control Groups
urinalysis
Immunomodulation
rats
Probiotics
hematology
Adrenal Glands
dosage
blood serum

Keywords

  • Lactobacillus kefiranofaciens M1
  • No-observed-adverse-effect-level
  • Probiotics
  • Sprague-Dawley rat
  • Toxicity

ASJC Scopus subject areas

  • Food Science
  • Toxicology
  • Medicine(all)

Cite this

Oral toxicity evaluation of kefir-isolated Lactobacillus kefiranofaciens M1 in Sprague-Dawley rats. / Owaga, E. E.; Chen, M. J.; Chen, W. Y.; Chen, C. W.; Hsieh, R. H.

In: Food and Chemical Toxicology, Vol. 70, 2014, p. 157-162.

Research output: Contribution to journalArticle

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