Oral inoculation of probiotics Lactobacillus acidophilus NCFM suppresses tumour growth both in segmental orthotopic colon cancer and extra-intestinal tissue

Chien Chang Chen, Wei Chuan Lin, Man Shan Kong, Hai Ning Shi, W. Allan Walker, Chun Yen Lin, Ching Tai Huang, Yung Chang Lin, Shih Ming Jung, Tzou Yien Lin

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Modulation of the cellular response by the administration of probiotic bacteria may be an effective strategy for preventing or inhibiting tumour growth. We orally pre-inoculated mice with probiotics Lactobacillus acidophilus NCFM (La) for 14d. Subcutaneous dorsal-flank tumours and segmental orthotopic colon cancers were implanted into mice using CT-26 murine colon adenocarcinoma cells. On day 28 after tumour initiation, the lamina propria of the colon, mesenteric lymph nodes (MLN) and spleen were harvested and purified for flow cytometry and mRNA analyses. We demonstrated that La pre-inoculation reduced tumour volume growth by 50•3%, compared with untreated mice at 28d after tumour implants (2465•5 (sem 1290•4) v. 4950•9 (sem 1689•3)mm3, P<0•001). Inoculation with La reduced the severity of colonic carcinogenesis caused by CT-26 cells, such as level of colonic involvement and structural abnormality of epithelial/crypt damage. Moreover, La enhanced apoptosis of CT-26 cells both in dorsal-flank tumour and segmental orthotopic colon cancer, and the mean counts of apoptotic body were higher in mice pre-inoculated with La (P<0•05) compared with untreated mice. La pre-inoculation down-regulated the CXCR4 mRNA expressions in the colon, MLN and extra-intestinal tissue, compared with untreated mice (P<0•05). In addition, La pre-inoculation reduced the mean fluorescence index of MHC class I (H-2Dd,-Kd and-Ld) in flow cytometry analysis. Taken together, these findings suggest that probiotics La may play a role in attenuating tumour growth during CT-26 cell carcinogenesis. The down-regulated expression of CXCR4 mRNA and MHC class I, as well as increasing apoptosis in tumour tissue, indicated that La may be associated with modulating the cellular response triggered by colon carcinogenesis.

Original languageEnglish
Pages (from-to)1623-1634
Number of pages12
JournalBritish Journal of Nutrition
Volume107
Issue number11
DOIs
Publication statusPublished - Jun 14 2012
Externally publishedYes

Fingerprint

Lactobacillus acidophilus
Probiotics
Colonic Neoplasms
Growth
Neoplasms
Colon
Carcinogenesis
Messenger RNA
Flow Cytometry
Lymph Nodes
Apoptosis
Tumor Burden
Mucous Membrane
Adenocarcinoma
Spleen
Fluorescence
Bacteria

Keywords

  • Apoptosis
  • Colon carcinogenesis
  • CT-26 cells
  • Lactobacillus acidophilus NCFM
  • Probiotics

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Oral inoculation of probiotics Lactobacillus acidophilus NCFM suppresses tumour growth both in segmental orthotopic colon cancer and extra-intestinal tissue. / Chen, Chien Chang; Lin, Wei Chuan; Kong, Man Shan; Shi, Hai Ning; Walker, W. Allan; Lin, Chun Yen; Huang, Ching Tai; Lin, Yung Chang; Jung, Shih Ming; Lin, Tzou Yien.

In: British Journal of Nutrition, Vol. 107, No. 11, 14.06.2012, p. 1623-1634.

Research output: Contribution to journalArticle

Chen, Chien Chang ; Lin, Wei Chuan ; Kong, Man Shan ; Shi, Hai Ning ; Walker, W. Allan ; Lin, Chun Yen ; Huang, Ching Tai ; Lin, Yung Chang ; Jung, Shih Ming ; Lin, Tzou Yien. / Oral inoculation of probiotics Lactobacillus acidophilus NCFM suppresses tumour growth both in segmental orthotopic colon cancer and extra-intestinal tissue. In: British Journal of Nutrition. 2012 ; Vol. 107, No. 11. pp. 1623-1634.
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