Oral cancer plasma tumor marker identified with bead-based affinity-fractionated proteomic technology

Ann Joy Cheng, Li Chiu Chen, Kun Yi Chien, Yin Ju Chen, Joseph Tung Chieh Chang, Hung Ming Wang, Chun Ta Liao, I. How Chen

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127 Citations (Scopus)

Abstract

Background: There is no plasma marker for detecting oral cancer, one of the most frequent cancers worldwide. We developed a bead-based affinity- fractionated proteomic method to discover a novel plasma marker for oral cancer. Methods: Affinity purification of heparinized plasma with magnetic beads and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis were used to screen potential oral cancer markers. We compiled MS protein profiles for 57 patients with oral cancer and compared them with profiles from 29 healthy controls. The spectra were analyzed statistically using flexAnalysis™ and Clin-Prot™ bioinformatic software. In each MS analysis, the peak intensities of interest were normalized with an internal standard (adrenocorticotropic hormone 18-39). For identification, affinity bead-purified plasma protein was subjected to MALDI TOF/TOF analysis followed by Mascot identification of the peptide sequences and a search of the National Center for Biotechnology Information protein database. Results: To optimize MALDI-TOF analysis based on the best discriminator of the cancer and control spectra, copper-chelated beads were used for plasma protein profiling. The within- and between-run CVs for assays were

Original languageEnglish
Pages (from-to)2236-2244
Number of pages9
JournalClinical Chemistry
Volume51
Issue number12
DOIs
Publication statusPublished - Dec 2005
Externally publishedYes

ASJC Scopus subject areas

  • Clinical Biochemistry

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    Cheng, A. J., Chen, L. C., Chien, K. Y., Chen, Y. J., Chang, J. T. C., Wang, H. M., Liao, C. T., & Chen, I. H. (2005). Oral cancer plasma tumor marker identified with bead-based affinity-fractionated proteomic technology. Clinical Chemistry, 51(12), 2236-2244. https://doi.org/10.1373/clinchem.2005.052324