Abstract
Platelet-rich plasma (PRP) containing autologous growth factors is applied in regenerative medicine, but the lack of an optimized PRP preparation protocol causes unstable therapeutic effects. The aim of this study was to optimize the PRP preparation method and compare the effects of PRP from different preparation methods in restoration of erectile function in a rat model. The in vivo experiments used Sprague-Dawley male rats (n=24), which were randomly divided into four groups of equal numbers: group I underwent sham operation, while the remaining three groups underwent bilateral CN crush. Crush injury groups were treated at the time of injury with an application of general PRP, optimized PRP [with the largest amount of platelet-derived growth factor (PDGF)-AB] or normal saline-only injection in the corpus cavernosum, respectively. Four weeks later, erectile function was assessed by CN electrosimulation, and penile tissue was collected for histology. Results demonstrated that in the PRP group prepared with the ACD-A anticoagulant, chitosan and incubated at -20°C for 15 days had the largest amount of PDGF-AB and showed a synergistic effect on release (p
| Original language | English |
|---|---|
| Journal | Journal of Tissue Engineering and Regenerative Medicine |
| DOIs | |
| Publication status | Accepted/In press - 2013 |
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Keywords
- Activating factor
- Cavernous nerve
- Erectile dysfunction
- Growth factor
- Intracavernous pressure
- Platelet-rich plasma
ASJC Scopus subject areas
- Biomaterials
- Medicine (miscellaneous)
- Biomedical Engineering
Cite this
Optimization of platelet-rich plasma and its effects on the recovery of erectile function after bilateral cavernous nerve injury in a rat model. / Wu, Yi No; Wu, Chien Chih; Sheu, Ming Thau; Chen, Kuo Chiang; Ho, Hsiu O.; Chiang, Han-Sun.
In: Journal of Tissue Engineering and Regenerative Medicine, 2013.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Optimization of platelet-rich plasma and its effects on the recovery of erectile function after bilateral cavernous nerve injury in a rat model
AU - Wu, Yi No
AU - Wu, Chien Chih
AU - Sheu, Ming Thau
AU - Chen, Kuo Chiang
AU - Ho, Hsiu O.
AU - Chiang, Han-Sun
PY - 2013
Y1 - 2013
N2 - Platelet-rich plasma (PRP) containing autologous growth factors is applied in regenerative medicine, but the lack of an optimized PRP preparation protocol causes unstable therapeutic effects. The aim of this study was to optimize the PRP preparation method and compare the effects of PRP from different preparation methods in restoration of erectile function in a rat model. The in vivo experiments used Sprague-Dawley male rats (n=24), which were randomly divided into four groups of equal numbers: group I underwent sham operation, while the remaining three groups underwent bilateral CN crush. Crush injury groups were treated at the time of injury with an application of general PRP, optimized PRP [with the largest amount of platelet-derived growth factor (PDGF)-AB] or normal saline-only injection in the corpus cavernosum, respectively. Four weeks later, erectile function was assessed by CN electrosimulation, and penile tissue was collected for histology. Results demonstrated that in the PRP group prepared with the ACD-A anticoagulant, chitosan and incubated at -20°C for 15 days had the largest amount of PDGF-AB and showed a synergistic effect on release (p
AB - Platelet-rich plasma (PRP) containing autologous growth factors is applied in regenerative medicine, but the lack of an optimized PRP preparation protocol causes unstable therapeutic effects. The aim of this study was to optimize the PRP preparation method and compare the effects of PRP from different preparation methods in restoration of erectile function in a rat model. The in vivo experiments used Sprague-Dawley male rats (n=24), which were randomly divided into four groups of equal numbers: group I underwent sham operation, while the remaining three groups underwent bilateral CN crush. Crush injury groups were treated at the time of injury with an application of general PRP, optimized PRP [with the largest amount of platelet-derived growth factor (PDGF)-AB] or normal saline-only injection in the corpus cavernosum, respectively. Four weeks later, erectile function was assessed by CN electrosimulation, and penile tissue was collected for histology. Results demonstrated that in the PRP group prepared with the ACD-A anticoagulant, chitosan and incubated at -20°C for 15 days had the largest amount of PDGF-AB and showed a synergistic effect on release (p
KW - Activating factor
KW - Cavernous nerve
KW - Erectile dysfunction
KW - Growth factor
KW - Intracavernous pressure
KW - Platelet-rich plasma
UR - http://www.scopus.com/inward/record.url?scp=84881522412&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84881522412&partnerID=8YFLogxK
U2 - 10.1002/term.1806
DO - 10.1002/term.1806
M3 - Article
JO - Journal of Tissue Engineering and Regenerative Medicine
JF - Journal of Tissue Engineering and Regenerative Medicine
SN - 1932-6254
ER -