Optimization of platelet-rich plasma and its effects on the recovery of erectile function after bilateral cavernous nerve injury in a rat model

Yi No Wu, Chien Chih Wu, Ming Thau Sheu, Kuo Chiang Chen, Hsiu O. Ho, Han-Sun Chiang

Research output: Contribution to journalArticle

Abstract

Platelet-rich plasma (PRP) containing autologous growth factors is applied in regenerative medicine, but the lack of an optimized PRP preparation protocol causes unstable therapeutic effects. The aim of this study was to optimize the PRP preparation method and compare the effects of PRP from different preparation methods in restoration of erectile function in a rat model. The in vivo experiments used Sprague-Dawley male rats (n=24), which were randomly divided into four groups of equal numbers: group I underwent sham operation, while the remaining three groups underwent bilateral CN crush. Crush injury groups were treated at the time of injury with an application of general PRP, optimized PRP [with the largest amount of platelet-derived growth factor (PDGF)-AB] or normal saline-only injection in the corpus cavernosum, respectively. Four weeks later, erectile function was assessed by CN electrosimulation, and penile tissue was collected for histology. Results demonstrated that in the PRP group prepared with the ACD-A anticoagulant, chitosan and incubated at -20°C for 15 days had the largest amount of PDGF-AB and showed a synergistic effect on release (p

Original languageEnglish
JournalJournal of Tissue Engineering and Regenerative Medicine
DOIs
Publication statusAccepted/In press - 2013

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Keywords

  • Activating factor
  • Cavernous nerve
  • Erectile dysfunction
  • Growth factor
  • Intracavernous pressure
  • Platelet-rich plasma

ASJC Scopus subject areas

  • Biomaterials
  • Medicine (miscellaneous)
  • Biomedical Engineering

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