Optical mapping of myocardial reactive oxygen species production throughout the reperfusion of global ischemia

Long Sheng Lu, Yen Bin Liu, Chia Wei Sun, Lung Chun Lin, Ming Jai Su, Chau Chung Wu

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Reactive oxygen species (ROS) are short-lived, highly reactive chemical entities that play significant roles in all levels of biology. However, their measurement requires destructive preparation, thereby limiting the continuous measurement of ROS in a living tissue. We develop an optical mapping system to visualize ROS production in an isolated and perfused rat heart. By staining the heart with dihydroethidium (DHE), a 532-nm laser beam is directed to the epicardial surface, where we collect the red fluorescence (>600 nm) for semiquantitative analysis. With this system, ROS production as well as ventricular pressure and ECG in isolated perfused rat hearts are monitored throughout the reperfusion of global ischemia. Ischemia would decrease myocardial ROS production, while reperfusion would immediately result in sustained ROS overproduction. Optical mapping would provide information regarding the spatial distribution and temporal evolution of myocardial ROS production, which would enhance knowledge of the role of free radicals in cardiovascular biology.

Original languageEnglish
Article number021012
JournalJournal of Biomedical Optics
Volume11
Issue number2
DOIs
Publication statusPublished - Mar 2006
Externally publishedYes

Fingerprint

ischemia
Reperfusion
Reactive Oxygen Species
Ischemia
Oxygen
oxygen
biology
rats
Rats
Optical Devices
Ventricular Pressure
staining
Electrocardiography
Free radicals
free radicals
Spatial distribution
Free Radicals
Laser beams
spatial distribution
Lasers

Keywords

  • Global ischemia
  • Optical mapping
  • Reactive oxygen species
  • Reperfusion

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology
  • Clinical Biochemistry

Cite this

Optical mapping of myocardial reactive oxygen species production throughout the reperfusion of global ischemia. / Lu, Long Sheng; Liu, Yen Bin; Sun, Chia Wei; Lin, Lung Chun; Su, Ming Jai; Wu, Chau Chung.

In: Journal of Biomedical Optics, Vol. 11, No. 2, 021012, 03.2006.

Research output: Contribution to journalArticle

Lu, Long Sheng ; Liu, Yen Bin ; Sun, Chia Wei ; Lin, Lung Chun ; Su, Ming Jai ; Wu, Chau Chung. / Optical mapping of myocardial reactive oxygen species production throughout the reperfusion of global ischemia. In: Journal of Biomedical Optics. 2006 ; Vol. 11, No. 2.
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