Oolong tea prevents cardiomyocyte loss against hypoxia by attenuating p-JNK mediated hypertrophy and enhancing P-IGF1R, p-akt, and p-Badser136 activity and by fortifying NRF2 antioxidation system

Marthandam Asokan Shibu, Chia Hua Kuo, Bih Cheng Chen, Da Tong Ju, Ray Jade Chen, Chao Hung Lai, Pei Jane Huang, Vijaya Padma Viswanadha, Wei Wen Kuo, Chih Yang Huang

Research output: Contribution to journalArticle

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Abstract

Tea, the most widely consumed natural beverage has been associated with reduced mortality risk from cardiovascular disease. Oolong tea is a partially fermented tea containing high levels of catechins, their degree of oxidation varies between 20%-80% causing differences in their active metabolites. In this study we examined the effect of oolong tea extract (OTE) obtained by oxidation at low-temperature for short-time against hypoxic injury and found that oolong tea provides cyto-protective effects by suppressing the JNK mediated hypertrophic effects and by enhancing the innate antioxidant mechanisms in neonatal cardiomyocytes and in H9c2 cells. OTE effectively attenuates 24 h hypoxia-triggered cardiomyocyte loss by suppressing caspase-3-cleavage and apoptosis in a dose-dependent manner. OTE also enhances the IGFIR/p-Akt associated survival-mechanism involving the elevation of p-Badser136 in a dose-dependent manner to aid cellular adaptations against hypoxic challenge. The results show the effects and mechanism of Oolong tea to provide cardio-protective benefits during hypoxic conditions.

Original languageEnglish
Pages (from-to)220-233
JournalEnvironmental Toxicology
Volume33
Issue number2
DOIs
Publication statusPublished - Feb 1 2018

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tea
Tea
hypoxia
Cardiac Myocytes
Hypertrophy
oxidation
mortality risk
Oxidation
Beverages
Hypoxia
loss
Catechin
cardiovascular disease
apoptosis
Metabolites
Caspase 3
antioxidant
cleavage
metabolite
Cardiovascular Diseases

Keywords

  • Cardiac apoptosis
  • Epigallocatechin gallate
  • Hypertrophy
  • Hypoxia
  • Oolong tea

ASJC Scopus subject areas

  • Toxicology
  • Management, Monitoring, Policy and Law
  • Health, Toxicology and Mutagenesis

Cite this

Oolong tea prevents cardiomyocyte loss against hypoxia by attenuating p-JNK mediated hypertrophy and enhancing P-IGF1R, p-akt, and p-Badser136 activity and by fortifying NRF2 antioxidation system. / Shibu, Marthandam Asokan; Kuo, Chia Hua; Chen, Bih Cheng; Ju, Da Tong; Chen, Ray Jade; Lai, Chao Hung; Huang, Pei Jane; Viswanadha, Vijaya Padma; Kuo, Wei Wen; Huang, Chih Yang.

In: Environmental Toxicology, Vol. 33, No. 2, 01.02.2018, p. 220-233.

Research output: Contribution to journalArticle

Shibu, Marthandam Asokan ; Kuo, Chia Hua ; Chen, Bih Cheng ; Ju, Da Tong ; Chen, Ray Jade ; Lai, Chao Hung ; Huang, Pei Jane ; Viswanadha, Vijaya Padma ; Kuo, Wei Wen ; Huang, Chih Yang. / Oolong tea prevents cardiomyocyte loss against hypoxia by attenuating p-JNK mediated hypertrophy and enhancing P-IGF1R, p-akt, and p-Badser136 activity and by fortifying NRF2 antioxidation system. In: Environmental Toxicology. 2018 ; Vol. 33, No. 2. pp. 220-233.
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