Abstract

One-pot green synthesis of fluorescent nitrogen-doped carbon nanodots (CNDs) was developed by hydrothermal treatments of biocompatible polyvinylpyrrolidone (PVP) and glycine. The fluorescent nitrogen-doped CNDs exhibited excellent water solubility, low cytotoxicity, and good salt stability for biological imaging. UV-vis spectroscopy, fluorescence spectroscopy, transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR) spectroscopy, and Raman spectroscopy were applied to confirm the optical and structural characteristics of the CNDs. Fluorescence of the CNDs was tunable from 417 to 450 nm adjusted by different excitation energy. Fluorescent quantum yield of the CNDs (21.43 %) was significantly increased ~47.59 % in comparison to that of the CNDs (14.52 %) without nitrogen doping by glycine. In the in vivo imaging system (IVIS), fluorescence signal of the nitrogen-doped CNDs was obviously observed in the lungs at 12- and 24-h post-injection. Our work has shown the potential applications of the nitrogen-doped CNDs in fluorescence imaging in vivo.

Original languageEnglish
Pages (from-to)77-82
Number of pages6
JournalAnalytical and Bioanalytical Chemistry
Volume408
Issue number1
DOIs
Publication statusPublished - Jan 1 2016

Fingerprint

Hydrothermal synthesis
Nitrogen
Carbon
Fluorescence
Optical Imaging
Glycine
Spectrum Analysis
Imaging techniques
Povidone
Raman Spectrum Analysis
Excitation energy
Fluorescence Spectrometry
Fluorescence spectroscopy
Quantum yield
Fourier Transform Infrared Spectroscopy
Cytotoxicity
Ultraviolet spectroscopy
Transmission Electron Microscopy
Imaging systems
Solubility

Keywords

  • Bioimaging
  • Carbon nanodots
  • Green synthesis
  • Nitrogen-doped

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

Cite this

One-pot green hydrothermal synthesis of fluorescent nitrogen-doped carbon nanodots for in vivo bioimaging. / Kuo, Tsung Rong; Sung, Shuo Yuan; Hsu, Chun Wei; Chang, Chih Jui; Chiu, Tai Chia; Hu, Cho Chun.

In: Analytical and Bioanalytical Chemistry, Vol. 408, No. 1, 01.01.2016, p. 77-82.

Research output: Contribution to journalArticle

Kuo, Tsung Rong ; Sung, Shuo Yuan ; Hsu, Chun Wei ; Chang, Chih Jui ; Chiu, Tai Chia ; Hu, Cho Chun. / One-pot green hydrothermal synthesis of fluorescent nitrogen-doped carbon nanodots for in vivo bioimaging. In: Analytical and Bioanalytical Chemistry. 2016 ; Vol. 408, No. 1. pp. 77-82.
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