Oncogenic Ras expression increases cytoplasmic distribution and phosphorylation of CSE1L in B16F10 melanoma cells

Pei Ru Wu, Chung Min Yeh, Chun Chi Chang, Hsuan Yuan Huang, Kai Cheng Wang, Hung Jen Shih, I. Yen Lee, Ming Chung Jiang, Yueh Min Lin

Research output: Contribution to journalArticle

Abstract

Ras activation confers the transformation activities of melanocytic lesions and malignant progression of melanoma. The CSE1L/CAS (chromosome segregation 1-like/cellular apoptosis susceptibility) gene is located in 20q13, a chromosomal region that correlates with melanoma development. CSE1L is highly expressed in melanoma and correlated with cancer stage and the poor prognosis of the disease. We studied the relation of Ras activation and the cytoplasmic distribution and phosphorylation of CAS in melanoma cells. Immunoblotting showed B16F10 melanoma cells overexpressing Ras increased CSE1L phosphorylation. Immunofluorescence showed that phosphorylated CSE1L mainly distributed in the cytoplasm of B16F10 melanoma cells, and Ras overexpression resulted in increased cytoplasmic distribution of phosphorylated CSE1L. CSE1L knockdown decreased the phosphorylation of the extracellular signal-regulated kinase1/2 (ERK1/2) induced by Ras in B16F10 melanoma cells. In immunohistochemistry, the tumor cells of melanoma showed strong cytoplasmic phosphor-CSE1L staining, while nevus cells showed weak cytoplasmic phosphor-CSE1L staining. Our results indicated that phosphorylated CSE1L plays a role in modulating the signaling pathway and progression of melanoma harboring Ras mutation.

Original languageEnglish
Pages (from-to)9889-9897
Number of pages9
JournalInternational Journal of Clinical and Experimental Pathology
Volume9
Issue number10
Publication statusPublished - Jan 1 2016
Externally publishedYes

Fingerprint

Melanoma
Phosphorylation
Staining and Labeling
Chromosome Segregation
Nevus
Chromosomes, Human, Pair 1
Immunoblotting
Fluorescent Antibody Technique
Neoplasms
Cytoplasm
Immunohistochemistry
Apoptosis
Mutation
Genes

Keywords

  • CAS
  • CSE1L
  • Cytoplasmic
  • Melanoma
  • Nuclear
  • Phosphorylation
  • Ras

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology

Cite this

Oncogenic Ras expression increases cytoplasmic distribution and phosphorylation of CSE1L in B16F10 melanoma cells. / Wu, Pei Ru; Yeh, Chung Min; Chang, Chun Chi; Huang, Hsuan Yuan; Wang, Kai Cheng; Shih, Hung Jen; Lee, I. Yen; Jiang, Ming Chung; Lin, Yueh Min.

In: International Journal of Clinical and Experimental Pathology, Vol. 9, No. 10, 01.01.2016, p. 9889-9897.

Research output: Contribution to journalArticle

Wu, Pei Ru ; Yeh, Chung Min ; Chang, Chun Chi ; Huang, Hsuan Yuan ; Wang, Kai Cheng ; Shih, Hung Jen ; Lee, I. Yen ; Jiang, Ming Chung ; Lin, Yueh Min. / Oncogenic Ras expression increases cytoplasmic distribution and phosphorylation of CSE1L in B16F10 melanoma cells. In: International Journal of Clinical and Experimental Pathology. 2016 ; Vol. 9, No. 10. pp. 9889-9897.
@article{689a851595454746ba71cb7115c15991,
title = "Oncogenic Ras expression increases cytoplasmic distribution and phosphorylation of CSE1L in B16F10 melanoma cells",
abstract = "Ras activation confers the transformation activities of melanocytic lesions and malignant progression of melanoma. The CSE1L/CAS (chromosome segregation 1-like/cellular apoptosis susceptibility) gene is located in 20q13, a chromosomal region that correlates with melanoma development. CSE1L is highly expressed in melanoma and correlated with cancer stage and the poor prognosis of the disease. We studied the relation of Ras activation and the cytoplasmic distribution and phosphorylation of CAS in melanoma cells. Immunoblotting showed B16F10 melanoma cells overexpressing Ras increased CSE1L phosphorylation. Immunofluorescence showed that phosphorylated CSE1L mainly distributed in the cytoplasm of B16F10 melanoma cells, and Ras overexpression resulted in increased cytoplasmic distribution of phosphorylated CSE1L. CSE1L knockdown decreased the phosphorylation of the extracellular signal-regulated kinase1/2 (ERK1/2) induced by Ras in B16F10 melanoma cells. In immunohistochemistry, the tumor cells of melanoma showed strong cytoplasmic phosphor-CSE1L staining, while nevus cells showed weak cytoplasmic phosphor-CSE1L staining. Our results indicated that phosphorylated CSE1L plays a role in modulating the signaling pathway and progression of melanoma harboring Ras mutation.",
keywords = "CAS, CSE1L, Cytoplasmic, Melanoma, Nuclear, Phosphorylation, Ras",
author = "Wu, {Pei Ru} and Yeh, {Chung Min} and Chang, {Chun Chi} and Huang, {Hsuan Yuan} and Wang, {Kai Cheng} and Shih, {Hung Jen} and Lee, {I. Yen} and Jiang, {Ming Chung} and Lin, {Yueh Min}",
year = "2016",
month = "1",
day = "1",
language = "English",
volume = "9",
pages = "9889--9897",
journal = "International Journal of Clinical and Experimental Pathology",
issn = "1936-2625",
publisher = "E-CENTURY PUBLISHING CORP",
number = "10",

}

TY - JOUR

T1 - Oncogenic Ras expression increases cytoplasmic distribution and phosphorylation of CSE1L in B16F10 melanoma cells

AU - Wu, Pei Ru

AU - Yeh, Chung Min

AU - Chang, Chun Chi

AU - Huang, Hsuan Yuan

AU - Wang, Kai Cheng

AU - Shih, Hung Jen

AU - Lee, I. Yen

AU - Jiang, Ming Chung

AU - Lin, Yueh Min

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Ras activation confers the transformation activities of melanocytic lesions and malignant progression of melanoma. The CSE1L/CAS (chromosome segregation 1-like/cellular apoptosis susceptibility) gene is located in 20q13, a chromosomal region that correlates with melanoma development. CSE1L is highly expressed in melanoma and correlated with cancer stage and the poor prognosis of the disease. We studied the relation of Ras activation and the cytoplasmic distribution and phosphorylation of CAS in melanoma cells. Immunoblotting showed B16F10 melanoma cells overexpressing Ras increased CSE1L phosphorylation. Immunofluorescence showed that phosphorylated CSE1L mainly distributed in the cytoplasm of B16F10 melanoma cells, and Ras overexpression resulted in increased cytoplasmic distribution of phosphorylated CSE1L. CSE1L knockdown decreased the phosphorylation of the extracellular signal-regulated kinase1/2 (ERK1/2) induced by Ras in B16F10 melanoma cells. In immunohistochemistry, the tumor cells of melanoma showed strong cytoplasmic phosphor-CSE1L staining, while nevus cells showed weak cytoplasmic phosphor-CSE1L staining. Our results indicated that phosphorylated CSE1L plays a role in modulating the signaling pathway and progression of melanoma harboring Ras mutation.

AB - Ras activation confers the transformation activities of melanocytic lesions and malignant progression of melanoma. The CSE1L/CAS (chromosome segregation 1-like/cellular apoptosis susceptibility) gene is located in 20q13, a chromosomal region that correlates with melanoma development. CSE1L is highly expressed in melanoma and correlated with cancer stage and the poor prognosis of the disease. We studied the relation of Ras activation and the cytoplasmic distribution and phosphorylation of CAS in melanoma cells. Immunoblotting showed B16F10 melanoma cells overexpressing Ras increased CSE1L phosphorylation. Immunofluorescence showed that phosphorylated CSE1L mainly distributed in the cytoplasm of B16F10 melanoma cells, and Ras overexpression resulted in increased cytoplasmic distribution of phosphorylated CSE1L. CSE1L knockdown decreased the phosphorylation of the extracellular signal-regulated kinase1/2 (ERK1/2) induced by Ras in B16F10 melanoma cells. In immunohistochemistry, the tumor cells of melanoma showed strong cytoplasmic phosphor-CSE1L staining, while nevus cells showed weak cytoplasmic phosphor-CSE1L staining. Our results indicated that phosphorylated CSE1L plays a role in modulating the signaling pathway and progression of melanoma harboring Ras mutation.

KW - CAS

KW - CSE1L

KW - Cytoplasmic

KW - Melanoma

KW - Nuclear

KW - Phosphorylation

KW - Ras

UR - http://www.scopus.com/inward/record.url?scp=84994578363&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84994578363&partnerID=8YFLogxK

M3 - Article

VL - 9

SP - 9889

EP - 9897

JO - International Journal of Clinical and Experimental Pathology

JF - International Journal of Clinical and Experimental Pathology

SN - 1936-2625

IS - 10

ER -