Oncogenic function and early detection potential of miRNA-10b in oral cancer as identified by microRNA profiling

Ya Ching Lu, Yin Ju Chen, Hung Ming Wang, Chi Ying Tsai, Wen Ho Chen, Yu Chen Huang, Kang Hsing Fan, Chi Neu Tsai, Shiang Fu Huang, Chung Jan Kang, Joseph Tung Chieh Chang, Ann Joy Cheng

Research output: Contribution to journalArticle

102 Citations (Scopus)

Abstract

The miRNA participates in a variety of biologic processes, and dysregulation of miRNA is associated with malignant transformation. In this study, we determined specific profile of miRNA associated with oral cancer by using miRNA array screening method. There were 23 miRNAs found with considerably differential expressions between six oral cancer cell lines and five lines of normal oral keratinocytes, in which, 10 miRNAs showed the highest significant difference after independent examination by reverse transcription quantitative PCR. Eight molecules were upregulated, miR-10b, miR-196a, miR-196b, miR-582-5p, miR-15b, miR-301, miR-148b, and miR-128a, and two molecules, miR-503 and miR-31, were downregulated. The most upregulated miR-10b was further examined, and its functions were characterized in two oral cancer cell lines. The miR-10b actively promotes cell migration (2.6- to 3.6-fold) and invasion (1.7- to 1.9-fold) but has minimal effect on cell growth or chemo-/radiosensitivity. Furthermore, miR-10b was considerably elevated in the plasma of xenografted tumor mice (20-fold). This upregulation of miR-10b in plasma was further shown in the patients with oral cancer [P <0.0001, area under curve (AUC) = 0.932] and precancer lesions (P <0.0001, AUC = 0.967), suggesting that miR-10b possesses a high potential to discriminate the normal subjects. In conclusion, we have identified at least 10 miRNAs significantly associated with oral cancer, including the most elevated miR-10b. The miR-10b actively participates in cancer formation by promoting cell migration and invasion. Our study using clinical samples suggests that plasma miR-10b has high potential as an early detection marker for oral cancer.

Original languageEnglish
Pages (from-to)665-674
Number of pages10
JournalCancer Prevention Research
Volume5
Issue number4
DOIs
Publication statusPublished - Apr 2012
Externally publishedYes

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Mouth Neoplasms
MicroRNAs
Area Under Curve
Cell Movement
Cell Line
Radiation Tolerance
Keratinocytes
Reverse Transcription
Neoplasms
Up-Regulation
Down-Regulation
Polymerase Chain Reaction
Growth

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Medicine(all)

Cite this

Oncogenic function and early detection potential of miRNA-10b in oral cancer as identified by microRNA profiling. / Lu, Ya Ching; Chen, Yin Ju; Wang, Hung Ming; Tsai, Chi Ying; Chen, Wen Ho; Huang, Yu Chen; Fan, Kang Hsing; Tsai, Chi Neu; Huang, Shiang Fu; Kang, Chung Jan; Chang, Joseph Tung Chieh; Cheng, Ann Joy.

In: Cancer Prevention Research, Vol. 5, No. 4, 04.2012, p. 665-674.

Research output: Contribution to journalArticle

Lu, YC, Chen, YJ, Wang, HM, Tsai, CY, Chen, WH, Huang, YC, Fan, KH, Tsai, CN, Huang, SF, Kang, CJ, Chang, JTC & Cheng, AJ 2012, 'Oncogenic function and early detection potential of miRNA-10b in oral cancer as identified by microRNA profiling', Cancer Prevention Research, vol. 5, no. 4, pp. 665-674. https://doi.org/10.1158/1940-6207.CAPR-11-0358
Lu, Ya Ching ; Chen, Yin Ju ; Wang, Hung Ming ; Tsai, Chi Ying ; Chen, Wen Ho ; Huang, Yu Chen ; Fan, Kang Hsing ; Tsai, Chi Neu ; Huang, Shiang Fu ; Kang, Chung Jan ; Chang, Joseph Tung Chieh ; Cheng, Ann Joy. / Oncogenic function and early detection potential of miRNA-10b in oral cancer as identified by microRNA profiling. In: Cancer Prevention Research. 2012 ; Vol. 5, No. 4. pp. 665-674.
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AU - Chen, Yin Ju

AU - Wang, Hung Ming

AU - Tsai, Chi Ying

AU - Chen, Wen Ho

AU - Huang, Yu Chen

AU - Fan, Kang Hsing

AU - Tsai, Chi Neu

AU - Huang, Shiang Fu

AU - Kang, Chung Jan

AU - Chang, Joseph Tung Chieh

AU - Cheng, Ann Joy

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