Abstract

Tubulointerstitial fibrosis is recognized as a key determinant of progressive chronic kidney disease (CKD). Fucoidan, a sulphated polysaccharide extracted from brown seaweed, exerts beneficial effects in some nephropathy models. The present study evaluated the inhibitory effect of oligo-fucoidan (800 Da) on renal tubulointerstitial fibrosis. We established a mouse CKD model by right nephrectomy with transient ischemic injury to the left kidney. Six weeks after the surgery, we fed the CKD mice oligo-fucoidan at 10, 20, and 100 mg/kg/d for 6 weeks and found that the oligo-fucoidan doses less than 100 mg/kg/d improved renal function and reduced renal tubulointerstitial fibrosis in CKD mice. Oligo-fucoidan also inhibited pressure-induced fibrotic responses and the expression of CD44, β-catenin, and TGF-β in rat renal tubular cells (NRK-52E). CD44 knockdown downregulated the expression of β-catenin and TGF-β in pressure-treated cells. Additional ligands for CD44 reduced the anti-fibrotic effect of oligo-fucoidan in NRK-52E cells. These data suggest that oligo-fucoidan at the particular dose prevents renal tubulointerstitial fibrosis in a CKD model. The anti-fibrotic effect of oligo-fucoidan may result from interfering with the interaction between CD44 and its extracellular ligands.

Original languageEnglish
Article number40183
JournalScientific Reports
Volume7
DOIs
Publication statusPublished - Jan 18 2017

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Signal Transduction
Fibrosis
Kidney
Chronic Renal Insufficiency
Catenins
Ligands
Pressure
Seaweed
fucoidan
Nephrectomy
Polysaccharides
Down-Regulation
Wounds and Injuries

ASJC Scopus subject areas

  • General

Cite this

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title = "Oligo-fucoidan prevents renal tubulointerstitial fibrosis by inhibiting the CD44 signal pathway",
abstract = "Tubulointerstitial fibrosis is recognized as a key determinant of progressive chronic kidney disease (CKD). Fucoidan, a sulphated polysaccharide extracted from brown seaweed, exerts beneficial effects in some nephropathy models. The present study evaluated the inhibitory effect of oligo-fucoidan (800 Da) on renal tubulointerstitial fibrosis. We established a mouse CKD model by right nephrectomy with transient ischemic injury to the left kidney. Six weeks after the surgery, we fed the CKD mice oligo-fucoidan at 10, 20, and 100 mg/kg/d for 6 weeks and found that the oligo-fucoidan doses less than 100 mg/kg/d improved renal function and reduced renal tubulointerstitial fibrosis in CKD mice. Oligo-fucoidan also inhibited pressure-induced fibrotic responses and the expression of CD44, β-catenin, and TGF-β in rat renal tubular cells (NRK-52E). CD44 knockdown downregulated the expression of β-catenin and TGF-β in pressure-treated cells. Additional ligands for CD44 reduced the anti-fibrotic effect of oligo-fucoidan in NRK-52E cells. These data suggest that oligo-fucoidan at the particular dose prevents renal tubulointerstitial fibrosis in a CKD model. The anti-fibrotic effect of oligo-fucoidan may result from interfering with the interaction between CD44 and its extracellular ligands.",
author = "Cheng-Hsien Chen and Yuh-Mou Sue and Chung-Yi Cheng and Chen, {Yen Cheng} and Liu, {Chung Te} and Yung-Ho Hsu and Hwang, {Pai An} and Huang, {Nai Jen} and Tso-Hsiao Chen",
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AU - Chen, Cheng-Hsien

AU - Sue, Yuh-Mou

AU - Cheng, Chung-Yi

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AU - Liu, Chung Te

AU - Hsu, Yung-Ho

AU - Hwang, Pai An

AU - Huang, Nai Jen

AU - Chen, Tso-Hsiao

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