Okadaic acid, a bioactive fatty acid from Halichondria okadai, stimulates lipolysis in rat adipocytes: The pivotal role of perilipin translocation

Nen Chung Chang, Aming Chor Ming Lin, Cheng Chen Hsu, Jung Sheng Liu, Leo Tsui, Chien Yuan Chen, Thanasekaran Jayakumar, Tsorng Harn Fong

Research output: Contribution to journalArticle

1 Citation (Scopus)


Lipid metabolism in visceral fat cells is correlated with metabolic syndrome and cardiovascular diseases. Okadaic-acid, a 38-carbon fatty acid isolated from the black sponge Halichondria okadai, can stimulate lipolysis by promoting the phosphorylation of several proteins in adipocytes. However, the mechanism of okadaic acid-induced lipolysis and the effects of okadaic acid on lipid-droplet-associated proteins (perilipins and beta-actin) remain unclear. We isolated adipocytes from rat epididymal fat pads and treated them with isoproterenol and/or okadaic acid to estimate lipolysis by measuring glycerol release. Incubating adipocytes with okadaic acid stimulated time-dependent lipolysis. Lipid-droplet-associated perilipins and beta-actin were analyzed by immunoblotting and immunofluorescence, and the association of perilipin A and B was found to be decreased in response to isoproterenol or okadaic acid treatment. Moreover, okadaic-acid treatment could enhance isoproterenol-mediated lipolysis, whereas treatment of several inhibitors such as KT-5720 (PKA inhibitor), calphostin C (PKC inhibitor), or KT-5823 (PKG inhibitor) did not attenuate okadaic-acid-induced lipolysis. By contrast, vanadyl acetylacetonate (tyrosine phosphatase inhibitor) blocked okadaic-acid-dependent lipolysis. These results suggest that okadaic acid induces the phosphorylation and detachment of lipid-droplet-associated perilipin A and B from the lipid droplet surface and thereby leads to accelerated lipolysis.

Original languageEnglish
Article number545739
JournalEvidence-based Complementary and Alternative Medicine
Publication statusPublished - 2013


ASJC Scopus subject areas

  • Complementary and alternative medicine

Cite this