Oct4 mediates tumor initiating properties in oral squamous cell carcinomas through the regulation of epithelial-mesenchymal transition

Lo Lin Tsai, Fang Wei Hu, Shiuan Shinn Lee, Chuan Hang Yu, Cheng Chia Yu, Yu Chao Chang

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Background: Overexpression of Oct4, an important transcription factor of embryonic stem cells (ESC), has been reported in several cancers. The aim of this study was to determine the emerging role of Oct4 in oral squamous cell carcinoma (OSCC) both in vitro and in vivo. Methodology/Principal Finding: Tumourigenic activity and molecular mechanisms of Oct4 overexpression or knockdown by lentiviral infection in OSCC was investigated in vitro and in vivo. Initially, we demonstrated that Oct4 expression was increased in OSCC cell lines as compared to a normal oral epithelial cell line SG. Overexpression of Oct4 was demonstrated to enhance cell proliferation, invasiveness, anchorage-independent growth and xenotransplantation tumourigenicity. These findings were coupled with epithelial-mesenchymal transition (EMT) transformation in OSCCs. In contrast, the silence of Oct4 significantly blocked the xenograft tumorigenesis of OSCC-derived cancer stem cells (OSCC-CSCs) and significantly improved the recipient survival. Clinically, the level of Oct4 expression was higher in recurrent and metastatic OSCC specimens but lower in primary OSCC specimens. Conclusion/Significance: Our results suggest that Oct4-mediated tumorigenecity is associated with the regulation of EMT. Oct4 might be a therapeutic target for OSCC.

Original languageEnglish
Article numbere87207
JournalPLoS One
Volume9
Issue number1
DOIs
Publication statusPublished - Jan 27 2014
Externally publishedYes

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Epithelial-Mesenchymal Transition
squamous cell carcinoma
Tumors
Squamous Cell Carcinoma
mouth
neoplasms
Neoplasms
Stem cells
cell lines
Cell Line
Heterologous Transplantation
Neoplastic Stem Cells
xenotransplantation
Embryonic Stem Cells
Epithelial Cells
Heterografts
embryonic stem cells
Cell proliferation
Carcinogenesis
carcinogenesis

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Oct4 mediates tumor initiating properties in oral squamous cell carcinomas through the regulation of epithelial-mesenchymal transition. / Tsai, Lo Lin; Hu, Fang Wei; Lee, Shiuan Shinn; Yu, Chuan Hang; Yu, Cheng Chia; Chang, Yu Chao.

In: PLoS One, Vol. 9, No. 1, e87207, 27.01.2014.

Research output: Contribution to journalArticle

Tsai, Lo Lin ; Hu, Fang Wei ; Lee, Shiuan Shinn ; Yu, Chuan Hang ; Yu, Cheng Chia ; Chang, Yu Chao. / Oct4 mediates tumor initiating properties in oral squamous cell carcinomas through the regulation of epithelial-mesenchymal transition. In: PLoS One. 2014 ; Vol. 9, No. 1.
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