Occurrence of D-amino acids and a pyridoxal 5′-phosphate-dependent aspartate racemase in the acidothermophilic archaeon, Thermoplasma acidophilum

Z. Long, J. A. Lee, T. Okamoto, M. Sekine, N. Nimura, K. Imai, M. Yohda, T. Maruyama, M. Sumi, N. Kamo, A. Yamagishi, T. Oshima, H. Homma

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Abstract

Free D-amino acid content in some archaea was investigated and D-forms of several amino acids were found in them. In the acidothermophilic archaeon, Thermoplasma acidophilum, the proportion of D-aspartate (D-Asp) to total Asp was as high as 39.7%. Crude extracts of Thermoplasma acidophilum had Asp-specific racemase activity that was pyridoxal 5′-phosphate (PLP)-dependent. The relative insensitivity to a SH-modifying reagent distinguished this activity from those of the PLP-independent Asp racemases found in other hyperthermophilic archaea (Matsumoto, M., et al., J. Bacteriol. 181, 6560-6563 1999). Thus, high levels of D-Asp should be produced by a new type(s) of Asp-specific racemase in Thermoplasma acidophilum, although the function of D-Asp in this archaeon remains unknown.

Original languageEnglish
Pages (from-to)317-321
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume281
Issue number2
DOIs
Publication statusPublished - 2001

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aspartate racemase
Thermoplasma
Racemases and Epimerases
D-Aspartic Acid
Pyridoxal Phosphate
Archaea
Amino Acids
Viperidae
Complex Mixtures
Sulfhydryl Reagents

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Occurrence of D-amino acids and a pyridoxal 5′-phosphate-dependent aspartate racemase in the acidothermophilic archaeon, Thermoplasma acidophilum. / Long, Z.; Lee, J. A.; Okamoto, T.; Sekine, M.; Nimura, N.; Imai, K.; Yohda, M.; Maruyama, T.; Sumi, M.; Kamo, N.; Yamagishi, A.; Oshima, T.; Homma, H.

In: Biochemical and Biophysical Research Communications, Vol. 281, No. 2, 2001, p. 317-321.

Research output: Contribution to journalArticle

Long, Z, Lee, JA, Okamoto, T, Sekine, M, Nimura, N, Imai, K, Yohda, M, Maruyama, T, Sumi, M, Kamo, N, Yamagishi, A, Oshima, T & Homma, H 2001, 'Occurrence of D-amino acids and a pyridoxal 5′-phosphate-dependent aspartate racemase in the acidothermophilic archaeon, Thermoplasma acidophilum', Biochemical and Biophysical Research Communications, vol. 281, no. 2, pp. 317-321. https://doi.org/10.1006/bbrc.2001.4353
Long, Z. ; Lee, J. A. ; Okamoto, T. ; Sekine, M. ; Nimura, N. ; Imai, K. ; Yohda, M. ; Maruyama, T. ; Sumi, M. ; Kamo, N. ; Yamagishi, A. ; Oshima, T. ; Homma, H. / Occurrence of D-amino acids and a pyridoxal 5′-phosphate-dependent aspartate racemase in the acidothermophilic archaeon, Thermoplasma acidophilum. In: Biochemical and Biophysical Research Communications. 2001 ; Vol. 281, No. 2. pp. 317-321.
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