Obstructive jaundice alters LFA-1α expression in rat small intestine

Shyr Ming Sheen-Chen, Han Shiang Chen, Hsin Tsung Ho, Chih Chi Sheen, Wei Jen Chen, Hock Liew Eng

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Translocation of bacteria and endotoxtin has long been documented in obstructive jaundice, and altered intestinal barrier function is considered to be one of the important mechanisms for this phenomenon. The regulation of gastrointestinal mucosal response to injury is thus of important clinical as well as biological relevance. Integrins play a critical role in enterocyte migration, which is essential to mucosal healing. This study is designed to evaluate the integrins status in obstructive jaundice. Male Sprague-Dawley rats (N = 37) were randomized to three groups. Group 1 (N = 12) underwent common bile duct ligation (CBDL), group 2 (N = 12) underwent common bile duct ligation with oral glutamine administration (CBDL + G), and group 3 (N = 13) underwent a sham operation (sham control). After seven days, segments of proximal jejunum and distal ileum were harvested, and cell surface immunohistochemical expression of LFA-1α and VLA-6 were evaluated and recorded. The staining intensities were graded on a scale of 0-4. Comparisons among the three groups were performed. There was no significant difference in VLA-6 staining on small intestine among the three groups (P > 0.05). There was also no significant difference in LFA-1α staining the on jejunum between group 1 (CBDL) and group 3 (sham control) (P > 0.05). However, the LFA-1α staining on the ileum in group 1 (CBDL) significantly decreased when compared with group 3 (sham control) (P = 0.008). With oral glutamine administration (0.2 g/kg body weight, once daily), LFA-1α staining on the ileum was significantly restored in group 2 (CBDL + G). In conclusion, obstructive jaundice for one week down-regulates LFA-1α expression on rat ileum. With oral glutamine administration, such down-regulation of LFA-1α expression on rat ileum can be restored. Such a phenomenon is intriguing and deserves further evaluation and elucidation.

Original languageEnglish
Pages (from-to)1165-1170
Number of pages6
JournalDigestive Diseases and Sciences
Volume48
Issue number6
DOIs
Publication statusPublished - Jun 1 2003
Externally publishedYes

Keywords

  • Glutamine
  • Integrin
  • LFA-1α
  • Obstructive jaundice
  • VLA-6

ASJC Scopus subject areas

  • Physiology
  • Gastroenterology

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