Obstructive jaundice alters kupffer cell function independent of bacterial translocation

Shyr Ming Sheen-Chen, Phuonglan Chau, Hobart W. Harris

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Background. There is a high incidence of perioperative morbidity and mortality in patients with obstructive jaundice. The absence of bile in the gastrointestinal tract promotes bacterial overgrowth and the increased translocation of bacteria and endotoxin to the liver which has been postulated to inhibit Kupffer cell function in these patients. But, biliary tract obstruction can directly damage liver cells and thus alter their function. Thus, we hypothesized that obstructive jaundice alone alters Kupffer cell function independent of the effects of bacterial translocation. This study was designed to evaluate the contribution of bacterial translocation to the altered Kupffer cell function observed in patients with obstructive jaundice. Methods. Sprague-Dawley rats were randomized to three groups of six animals each. Group 1 underwent common bile duct ligation with intestinal bile salt replacement (sodium taurocholate 100 mg/kg/day) via gastrostomy and an implantable osmotic pump (CBDL + bile salts), Group 2 underwent common bile duct ligation with normal saline replacement (CBDL + saline), and Group 3 underwent a sham operation (sham control). After 7 days, tissue and blood were collected for bacterial translocation and biochemical analyses. Examination of cultured Kupffer cell function included measuring the phagocytosis of heat-killed Candida albicans and endotoxin (LPS)-induced TNFα and nitric oxide production. Results. While bacterial translocation and cecal bacterial counts were significantly increased in the CBDL + saline group, these parameters were both reduced to control levels following intestinal bile salt replacement (CBDL + bile salts). Altered Kupffer cell function, as measured by the increased phagocytosis of C. albicans and LPS- induced NO production, and decreased LPS-induced TNFα production were observed in all animals with obstructive jaundice regardless of bile salt replacement. Conclusion. Kupffer cell function appears to be differentially affected by obstructive jaundice and these altered functions can occur independent of bacterial translocation.

Original languageEnglish
Pages (from-to)205-209
Number of pages5
JournalJournal of Surgical Research
Volume80
Issue number2
DOIs
Publication statusPublished - Dec 1998
Externally publishedYes

Fingerprint

Bacterial Translocation
Kupffer Cells
Obstructive Jaundice
Bile Acids and Salts
Common Bile Duct
Candida albicans
Phagocytosis
Endotoxins
Ligation
Taurocholic Acid
Gastrostomy
Bacterial Load
Liver
Biliary Tract
Bile
Sprague Dawley Rats
Gastrointestinal Tract
Cultured Cells
Nitric Oxide
Hot Temperature

Keywords

  • Bile salts
  • Endotoxin
  • Liver
  • Macrophage

ASJC Scopus subject areas

  • Surgery

Cite this

Obstructive jaundice alters kupffer cell function independent of bacterial translocation. / Sheen-Chen, Shyr Ming; Chau, Phuonglan; Harris, Hobart W.

In: Journal of Surgical Research, Vol. 80, No. 2, 12.1998, p. 205-209.

Research output: Contribution to journalArticle

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abstract = "Background. There is a high incidence of perioperative morbidity and mortality in patients with obstructive jaundice. The absence of bile in the gastrointestinal tract promotes bacterial overgrowth and the increased translocation of bacteria and endotoxin to the liver which has been postulated to inhibit Kupffer cell function in these patients. But, biliary tract obstruction can directly damage liver cells and thus alter their function. Thus, we hypothesized that obstructive jaundice alone alters Kupffer cell function independent of the effects of bacterial translocation. This study was designed to evaluate the contribution of bacterial translocation to the altered Kupffer cell function observed in patients with obstructive jaundice. Methods. Sprague-Dawley rats were randomized to three groups of six animals each. Group 1 underwent common bile duct ligation with intestinal bile salt replacement (sodium taurocholate 100 mg/kg/day) via gastrostomy and an implantable osmotic pump (CBDL + bile salts), Group 2 underwent common bile duct ligation with normal saline replacement (CBDL + saline), and Group 3 underwent a sham operation (sham control). After 7 days, tissue and blood were collected for bacterial translocation and biochemical analyses. Examination of cultured Kupffer cell function included measuring the phagocytosis of heat-killed Candida albicans and endotoxin (LPS)-induced TNFα and nitric oxide production. Results. While bacterial translocation and cecal bacterial counts were significantly increased in the CBDL + saline group, these parameters were both reduced to control levels following intestinal bile salt replacement (CBDL + bile salts). Altered Kupffer cell function, as measured by the increased phagocytosis of C. albicans and LPS- induced NO production, and decreased LPS-induced TNFα production were observed in all animals with obstructive jaundice regardless of bile salt replacement. Conclusion. Kupffer cell function appears to be differentially affected by obstructive jaundice and these altered functions can occur independent of bacterial translocation.",
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