Numb is involved in the non-random segregation of subcellular vesicles in colorectal cancer stem cells

Wei Lun Hwang, Muh Hwa Yang

Research output: Contribution to journalReview article

7 Citations (Scopus)

Abstract

The balance between the symmetric and asymmetric division of stem cells governs tissue homeostasis, and the deregulation of this balance initiates tumor formation. Although many functions of Numb have been demonstrated in normal stem cells, the role of Numb in cancer stem cells is relatively unclear. We recently demonstrated that in colorectal cancer stem cells, Numb was suppressed by miR-146a-5p, which resulted in the activation of the Wnt signaling pathway and symmetric template DNA division. Here, we demonstrate that the PKH26-labeled subcellular foci are enriched for endosomal markers such as EEA1 and RAB11. In colorectal cancer stem cells, the PKH-26-labeled vesicles are segregated equally at the first mitotic division; in contrast, they are unequally segregated in parental cells or in cancer stem cells undergoing serum-induced differentiation. The PKHBright progeny of colorectal cancer stem cells harbors a stem cell phenotype, whereas the PKHDim progeny behaves as the differentiating cells. The miR-146a-5p-regulated Numb controls the distribution of PKH26 vesicles. Our results suggest a critical role of Numb in controlling the segregation of subcellular vesicles during division of colorectal cancer stem cells.

Original languageEnglish
Pages (from-to)2697-2703
Number of pages7
JournalCell Cycle
Volume15
Issue number20
DOIs
Publication statusPublished - Oct 17 2016

Keywords

  • Asymmetric Cell Division
  • Cancer Stem Cell
  • Colorectal Cancer
  • miRNA
  • Numb

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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