Nuclear translocation of cytochrome c during apoptosis

Alam Nur-E-Kamal, Stephane R. Gross, Zui Pan, Zita Balklava, Jianjie Ma, Leroy-Fong Liu

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Abstract

Release of cytochrome c from mitochondria is a major event during apoptosis. Released cytochrome c has been shown to activate caspase-dependent apoptotic signals. In this report, we provide evidence for a novel role of cytochrome c in caspase-independent nuclear apoptosis. We showed that cytochrome c, released from mitochondria upon apoptosis induction, gradually accumulates in the nucleus as evidenced by both immunofluorescence and subcellular fractionation. Parallel to nuclear accumulation of cytochrome c, acetylated histone H2A, but not unmodified H2A, was released from the nucleus to the cytoplasm. Addition of purified cytochrome c to isolated nuclei recapitulated the preferential release of acetylated, but not deacetylated, histone H2A. Cytochrome c was also found to induce chromatin condensation. These results suggest that the nuclear accumulation of cytochrome c may be directly involved in the remodeling of chromatin. Our results provide evidence of a novel role for cytochrome c in inducing nuclear apoptosis.

Original languageEnglish
Pages (from-to)24911-24914
Number of pages4
JournalJournal of Biological Chemistry
Volume279
Issue number24
DOIs
Publication statusPublished - Jun 11 2004
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry

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    Nur-E-Kamal, A., Gross, S. R., Pan, Z., Balklava, Z., Ma, J., & Liu, L-F. (2004). Nuclear translocation of cytochrome c during apoptosis. Journal of Biological Chemistry, 279(24), 24911-24914. https://doi.org/10.1074/jbc.C400051200