Nuclear factor Y regulation and promoter transactivation of human ribonucleotide reductase subunit M2 gene in a Gemcitabine resistant KB clone

Xiyong Liu, Bingsen Zhou, Lijun Xue, Weihua Qiu, Jennifer Shih, Shu Zheng, Yun Yen

Research output: Contribution to journalArticle

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Abstract

Overexpression of human ribonucleotide reductase subunit M2 (hRRM2) has been shown as a potential factor causing Gemcitabine (Gem) resistance. We hypothesized the nuclear factor Y (NF-Y) would transcriptionally regulate hRRM2 and contribute to overexpression of hRRM2 in a Gem resistant clone. A luciferase and gel shift assay, and a Southwestern blot were employed to analyze the promoter activity of hRRM2. The data exhibited the hRRM2 promoter was upregulated almost 5-fold in the Gem resistant KB clone (KBGem) via three sequential CCAAT boxes located in the proximal promoter region. Nuclear extracts from KB and KBGem could interact with the CCAAT motif of the hRRM2 proximal promoter region, and could form DNA-protein complexes with different binding patterns. The complexes could be further recognized with antibodies against NF-Y subunits A and B. Histone deacetylases (HDAC) involvement in NF-Y transcription repression in the KBGem clone was examined. A HDAC activity assay revealed a 3-fold decrease of HDAC activity in the KBGem clone compared to KB cells. Parental cells were treated with trichostatin A (TSA), a HDAC inhibitor. NF-Y transactivation was induced, resulting in an increase of hRRM2 expression. This led to an expanded dCTP pool and an abrogated [3H]Gemcitabine incorporation. In addition, microarray analysis results showed most of the proliferation-related genes were upregulated in KBGem. This finding was consistent with enhanced NF-Y transactivation in KBGem. In summary, upregulation of NF-Y transactivation increased hRRM2 transcription, which played a pivotal role in the Gem resistant KB clone.

Original languageEnglish
Pages (from-to)1499-1511
Number of pages13
JournalBiochemical Pharmacology
Volume67
Issue number8
DOIs
Publication statusPublished - Apr 15 2004
Externally publishedYes

Fingerprint

gemcitabine
Transcriptional Activation
Clone Cells
Genes
Histone Deacetylases
Transcription
Genetic Promoter Regions
Assays
trichostatin A
Southwestern Blotting
KB Cells
nuclear factor Y
ribonucleotide reductase M2
Microarray Analysis
Microarrays
Luciferases
Human Activities

Keywords

  • hRR
  • hRRM1
  • hRRM2
  • Human ribonucleotide reductase
  • Human ribonucleotide reductase subunit M1
  • Human ribonucleotide reductase subunit M2
  • KBGem
  • NF-Y
  • Nuclear factor Y
  • p53 dependant ribonucleotide reductase subunit R2
  • p53R2

ASJC Scopus subject areas

  • Biochemistry
  • Pharmacology

Cite this

Nuclear factor Y regulation and promoter transactivation of human ribonucleotide reductase subunit M2 gene in a Gemcitabine resistant KB clone. / Liu, Xiyong; Zhou, Bingsen; Xue, Lijun; Qiu, Weihua; Shih, Jennifer; Zheng, Shu; Yen, Yun.

In: Biochemical Pharmacology, Vol. 67, No. 8, 15.04.2004, p. 1499-1511.

Research output: Contribution to journalArticle

Liu, Xiyong ; Zhou, Bingsen ; Xue, Lijun ; Qiu, Weihua ; Shih, Jennifer ; Zheng, Shu ; Yen, Yun. / Nuclear factor Y regulation and promoter transactivation of human ribonucleotide reductase subunit M2 gene in a Gemcitabine resistant KB clone. In: Biochemical Pharmacology. 2004 ; Vol. 67, No. 8. pp. 1499-1511.
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AU - Xue, Lijun

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AU - Shih, Jennifer

AU - Zheng, Shu

AU - Yen, Yun

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AB - Overexpression of human ribonucleotide reductase subunit M2 (hRRM2) has been shown as a potential factor causing Gemcitabine (Gem) resistance. We hypothesized the nuclear factor Y (NF-Y) would transcriptionally regulate hRRM2 and contribute to overexpression of hRRM2 in a Gem resistant clone. A luciferase and gel shift assay, and a Southwestern blot were employed to analyze the promoter activity of hRRM2. The data exhibited the hRRM2 promoter was upregulated almost 5-fold in the Gem resistant KB clone (KBGem) via three sequential CCAAT boxes located in the proximal promoter region. Nuclear extracts from KB and KBGem could interact with the CCAAT motif of the hRRM2 proximal promoter region, and could form DNA-protein complexes with different binding patterns. The complexes could be further recognized with antibodies against NF-Y subunits A and B. Histone deacetylases (HDAC) involvement in NF-Y transcription repression in the KBGem clone was examined. A HDAC activity assay revealed a 3-fold decrease of HDAC activity in the KBGem clone compared to KB cells. Parental cells were treated with trichostatin A (TSA), a HDAC inhibitor. NF-Y transactivation was induced, resulting in an increase of hRRM2 expression. This led to an expanded dCTP pool and an abrogated [3H]Gemcitabine incorporation. In addition, microarray analysis results showed most of the proliferation-related genes were upregulated in KBGem. This finding was consistent with enhanced NF-Y transactivation in KBGem. In summary, upregulation of NF-Y transactivation increased hRRM2 transcription, which played a pivotal role in the Gem resistant KB clone.

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