Novel codon-optimized GM-CSF gene as an adjuvant to enhance the immunity of a DNA vaccine against HIV-1 Gag

Jian Tai Qiu, Ting Chang Chang, Cheng Tao Lin, Yi Ming Chen, Frank Q. Li, Yung Kuei Soong, Chyong Huey Lai

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a potent immunomodulatory cytokine. Here we generated a novel codon-optimized murine GM-CSF gene as an adjuvant. The codon-optimized GM-CSF gene significantly increased protein expression levels in all cells tested. Although injection of the wild-type GM-CSF plasmids adjuvanted HIV-1 Gag DNA vaccine induced detectable immune responses, co-administration of plasmids encoding the codon-optimized GM-CSF sequence with the DNA vaccine resulted in a strong antibody and CTL responses and a protective immune response against infection with recombinant vaccinia virus expressing HIV-1 Gag. This novel codon-optimized GM-CSF gene offers a practical molecular strategy for potentiating immune responses to vaccines as well as other immunotherapeutic strategies.

Original languageEnglish
Pages (from-to)253-263
Number of pages11
JournalVaccine
Volume25
Issue number2
DOIs
Publication statusPublished - Jan 4 2007

Keywords

  • DNA vaccine
  • GM-CSF
  • HIV-1
  • Immunity
  • T-lymphocytes

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Fingerprint Dive into the research topics of 'Novel codon-optimized GM-CSF gene as an adjuvant to enhance the immunity of a DNA vaccine against HIV-1 Gag'. Together they form a unique fingerprint.

  • Cite this