Novel codon-optimized GM-CSF gene as an adjuvant to enhance the immunity of a DNA vaccine against HIV-1 Gag

Jian Tai Qiu, Ting Chang Chang, Cheng Tao Lin, Yi Ming Chen, Frank Q. Li, Yung Kuei Soong, Chyong Huey Lai

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a potent immunomodulatory cytokine. Here we generated a novel codon-optimized murine GM-CSF gene as an adjuvant. The codon-optimized GM-CSF gene significantly increased protein expression levels in all cells tested. Although injection of the wild-type GM-CSF plasmids adjuvanted HIV-1 Gag DNA vaccine induced detectable immune responses, co-administration of plasmids encoding the codon-optimized GM-CSF sequence with the DNA vaccine resulted in a strong antibody and CTL responses and a protective immune response against infection with recombinant vaccinia virus expressing HIV-1 Gag. This novel codon-optimized GM-CSF gene offers a practical molecular strategy for potentiating immune responses to vaccines as well as other immunotherapeutic strategies.

Original languageEnglish
Pages (from-to)253-263
Number of pages11
JournalVaccine
Volume25
Issue number2
DOIs
Publication statusPublished - Jan 4 2007
Externally publishedYes

Fingerprint

granulocyte-macrophage colony-stimulating factor
DNA Vaccines
recombinant vaccines
Granulocyte-Macrophage Colony-Stimulating Factor
Human immunodeficiency virus 1
codons
Codon
adjuvants
HIV-1
Immunity
immunity
Genes
genes
immune response
plasmids
Plasmids
Vaccinia virus
Antibody Formation
cytokines
Vaccines

Keywords

  • DNA vaccine
  • GM-CSF
  • HIV-1
  • Immunity
  • T-lymphocytes

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Cite this

Novel codon-optimized GM-CSF gene as an adjuvant to enhance the immunity of a DNA vaccine against HIV-1 Gag. / Qiu, Jian Tai; Chang, Ting Chang; Lin, Cheng Tao; Chen, Yi Ming; Li, Frank Q.; Soong, Yung Kuei; Lai, Chyong Huey.

In: Vaccine, Vol. 25, No. 2, 04.01.2007, p. 253-263.

Research output: Contribution to journalArticle

Qiu, Jian Tai ; Chang, Ting Chang ; Lin, Cheng Tao ; Chen, Yi Ming ; Li, Frank Q. ; Soong, Yung Kuei ; Lai, Chyong Huey. / Novel codon-optimized GM-CSF gene as an adjuvant to enhance the immunity of a DNA vaccine against HIV-1 Gag. In: Vaccine. 2007 ; Vol. 25, No. 2. pp. 253-263.
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