Noninvasive imaging of reporter gene expression and distribution in vivo

Kuo Hsiang Chuang, Tian L. Cheng

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Repeated noninvasive imaging of reporter gene expression is increasingly used for monitoring the expression and location of genes and cells in experimental animals and humans. Development of transgenic animals that express reporter genes in specific tissues/organs or the whole body possess the potential for understanding the course of disease or serve as a syngenic cell/tissue/organ donor to study transplant survival in recipients. In this review, we discuss the major reporter genes used in gene therapy, cell therapy and transgenic animals, including exogenous reporters (herpes simplex virus type 1 thymidine kinase, fluorescent proteins and luciferase) and endogenous reporters (dopamine 2 receptor, transferrin receptor, sodium iodide symporter, β-glucuronidase, and antibody reporter). The clinical potential of these reporter genes is also discussed with regard to their imaging specificity and immunogenicity in humans.

Original languageEnglish
Pages (from-to)1-11
Number of pages11
JournalFooyin Journal of Health Sciences
Volume2
Issue number1
DOIs
Publication statusPublished - Feb 2010
Externally publishedYes

Fingerprint

Reporter Genes
Gene expression
Genes
Gene Expression
Imaging techniques
Genetically Modified Animals
Animals
Tissue Donors
Tissue
Gene therapy
Transplants
Transferrin Receptors
Thymidine Kinase
Glucuronidase
Dopamine Receptors
Human Herpesvirus 1
Gene Expression Profiling
Cell- and Tissue-Based Therapy
Luciferases
Viruses

Keywords

  • Endogenous reporter
  • Exogenous reporter
  • Noninvasive imaging
  • Reporter gene

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biomedical Engineering
  • Medicine(all)
  • Drug Discovery

Cite this

Noninvasive imaging of reporter gene expression and distribution in vivo. / Chuang, Kuo Hsiang; Cheng, Tian L.

In: Fooyin Journal of Health Sciences, Vol. 2, No. 1, 02.2010, p. 1-11.

Research output: Contribution to journalArticle

@article{d210cd3af7ff4ae1a01f1d54d919e2ed,
title = "Noninvasive imaging of reporter gene expression and distribution in vivo",
abstract = "Repeated noninvasive imaging of reporter gene expression is increasingly used for monitoring the expression and location of genes and cells in experimental animals and humans. Development of transgenic animals that express reporter genes in specific tissues/organs or the whole body possess the potential for understanding the course of disease or serve as a syngenic cell/tissue/organ donor to study transplant survival in recipients. In this review, we discuss the major reporter genes used in gene therapy, cell therapy and transgenic animals, including exogenous reporters (herpes simplex virus type 1 thymidine kinase, fluorescent proteins and luciferase) and endogenous reporters (dopamine 2 receptor, transferrin receptor, sodium iodide symporter, β-glucuronidase, and antibody reporter). The clinical potential of these reporter genes is also discussed with regard to their imaging specificity and immunogenicity in humans.",
keywords = "Endogenous reporter, Exogenous reporter, Noninvasive imaging, Reporter gene",
author = "Chuang, {Kuo Hsiang} and Cheng, {Tian L.}",
year = "2010",
month = "2",
doi = "10.1016/S1877-8607(10)60007-0",
language = "English",
volume = "2",
pages = "1--11",
journal = "Genomic Medicine, Biomarkers, and Health Sciences",
issn = "2214-0247",
publisher = "Elsevier BV",
number = "1",

}

TY - JOUR

T1 - Noninvasive imaging of reporter gene expression and distribution in vivo

AU - Chuang, Kuo Hsiang

AU - Cheng, Tian L.

PY - 2010/2

Y1 - 2010/2

N2 - Repeated noninvasive imaging of reporter gene expression is increasingly used for monitoring the expression and location of genes and cells in experimental animals and humans. Development of transgenic animals that express reporter genes in specific tissues/organs or the whole body possess the potential for understanding the course of disease or serve as a syngenic cell/tissue/organ donor to study transplant survival in recipients. In this review, we discuss the major reporter genes used in gene therapy, cell therapy and transgenic animals, including exogenous reporters (herpes simplex virus type 1 thymidine kinase, fluorescent proteins and luciferase) and endogenous reporters (dopamine 2 receptor, transferrin receptor, sodium iodide symporter, β-glucuronidase, and antibody reporter). The clinical potential of these reporter genes is also discussed with regard to their imaging specificity and immunogenicity in humans.

AB - Repeated noninvasive imaging of reporter gene expression is increasingly used for monitoring the expression and location of genes and cells in experimental animals and humans. Development of transgenic animals that express reporter genes in specific tissues/organs or the whole body possess the potential for understanding the course of disease or serve as a syngenic cell/tissue/organ donor to study transplant survival in recipients. In this review, we discuss the major reporter genes used in gene therapy, cell therapy and transgenic animals, including exogenous reporters (herpes simplex virus type 1 thymidine kinase, fluorescent proteins and luciferase) and endogenous reporters (dopamine 2 receptor, transferrin receptor, sodium iodide symporter, β-glucuronidase, and antibody reporter). The clinical potential of these reporter genes is also discussed with regard to their imaging specificity and immunogenicity in humans.

KW - Endogenous reporter

KW - Exogenous reporter

KW - Noninvasive imaging

KW - Reporter gene

UR - http://www.scopus.com/inward/record.url?scp=77955246880&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77955246880&partnerID=8YFLogxK

U2 - 10.1016/S1877-8607(10)60007-0

DO - 10.1016/S1877-8607(10)60007-0

M3 - Article

AN - SCOPUS:77955246880

VL - 2

SP - 1

EP - 11

JO - Genomic Medicine, Biomarkers, and Health Sciences

JF - Genomic Medicine, Biomarkers, and Health Sciences

SN - 2214-0247

IS - 1

ER -