Nonhistone proteins HMG1 and HMG2 unwind DNA double helix

Kashayar Javaherian, Mohamad Sadeghi, Leroy-Fong Liu

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

In a previous communication we have shown that both HMG1 and HMG2 non-histone proteins change the DNA helical structure and the binding of HMG1 and HMG2 to DNA induces a net unwinding equivalent of DNA double helix (Javaherian, K., Liu, L. F. and Wang, J. C. (1978) Science, 199, 1345-1346). Employing melting absorption technique, we now show that in the presence of salt HMG1 and HMG2 destabilize DNA whereas in the absence of salt, they both stabilize DNA molecules. Consequently the folded structure of HMG must play an important role in melting DNA. Furthermore, by measuring topological winding number using competition unwinding experiments, we conclude that HMG1 has a higher affinity for a single-stranded DNA relative to double-stranded DNA. These results together suggest that HMG1 and HMG2 unwind DNA double helix by local denaturation of the DNA base pairs. The net unwinding angles have been measured to be 22° and 26° per molecule of HMG1 and HMG2 respectively.

Original languageEnglish
Pages (from-to)3569-3580
Number of pages12
JournalNucleic Acids Research
Volume6
Issue number11
DOIs
Publication statusPublished - Aug 10 1979
Externally publishedYes

Fingerprint

HMGB2 Protein
HMGB1 Protein
Helix
Melting
Salt
DNA
Molecules
Proteins
Protein
Winding number
Affine transformation
Absorption
Nucleic Acid Denaturation
Angle
Experiment
Salts
Single-Stranded DNA
Base Pairing
Denaturation
Freezing

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

Nonhistone proteins HMG1 and HMG2 unwind DNA double helix. / Javaherian, Kashayar; Sadeghi, Mohamad; Liu, Leroy-Fong.

In: Nucleic Acids Research, Vol. 6, No. 11, 10.08.1979, p. 3569-3580.

Research output: Contribution to journalArticle

Javaherian, Kashayar ; Sadeghi, Mohamad ; Liu, Leroy-Fong. / Nonhistone proteins HMG1 and HMG2 unwind DNA double helix. In: Nucleic Acids Research. 1979 ; Vol. 6, No. 11. pp. 3569-3580.
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