核子醫學分子造影於基因治療之應用

Translated title of the contribution: Non-Invasive Mapping of Gene Expression for Gene Therapy with Molecular Nuclear Medicine Imaging

鄧 文炳(Win-Ping Deng), Chih-Hsien Chang, 賴 文福(Wen-Fu Lai), Hsin-Ell Wang, Gann Ting, Wen K. Yang, Ren-Shyan Liu

Research output: Contribution to journalArticle

Abstract

The increasing knowledge of the molecular biology and molecular genetics of human diseases, particularly in human cancer, has led to the application of new biological therapeutic approaches such as gene-based therapy. A limitation of many gene-based therapies has been the inability to achieve controlled and effective delivery of gene to the target cells or tissues. In addition, several issues that are important for clinical optimization of gene therapy remain unresolved: (a) whether gene transduction has been successful; (b) what is the distribution of transduced target cells; (c) whether the level of gene expression is adequate. A noninvasive, clinically applicable method for imaging gene transduction in target tissue or specific organs of the body, thus assessing the kinetic changes of functional expression of the therapeutic gene, would be of considerable value. This paper reviewed and discussed the successful clinical application of this method to monitor gene expression which will require the appropriate combination of marker gene and marker substrate. This will facilitate the monitoring and evaluation of gene therapy in human subjects by defining the location, magnitude, and persistence of gene expression over time.
Translated title of the contributionNon-Invasive Mapping of Gene Expression for Gene Therapy with Molecular Nuclear Medicine Imaging
Original languageTraditional Chinese
Pages (from-to)237-246
Number of pages10
Journal核子醫學雜誌
Volume14
Issue number4
DOIs
Publication statusPublished - 2001

Keywords

  • non-invasive
  • nuclear medicine imaging
  • cancer therapy
  • gene therapy
  • PET
  • SPECT
  • herpes simplex virus
  • thymidine kinase
  • marker substrate
  • marker gene
  • reporter gene

Fingerprint Dive into the research topics of 'Non-Invasive Mapping of Gene Expression for Gene Therapy with Molecular Nuclear Medicine Imaging'. Together they form a unique fingerprint.

  • Cite this

    鄧文炳(Win-Ping D, Chang, C-H., 賴文福(Wen-Fu L, Wang, H-E., Ting, G., Yang, W. K., & Liu, R-S. (2001). 核子醫學分子造影於基因治療之應用. 核子醫學雜誌, 14(4), 237-246. https://doi.org/10.6332/ANMS.1404.012